The transfection assay is an important tool for evaluating the health effects of industrial chemicals, with the reporter gene expression as an indicator. However, heavy metals often influence the expression of the reference plasmids used to correct variations in transfection efficiency between assay plates, reducing the reliability of this assay. We found that the target of heavy metals is the ...

The rate of consumption of dithiothreitol (DTT) is increasingly used to measure the oxidative potential of particulate matter (PM), which has been linked to the adverse health effects of PM. While several quinones are known to be very reactive in the DTT assay, it is unclear what other chemical species might contribute to the loss of DTT in PM extracts. To address this question, we quantify the...

The protease inhibitive assay for heavy metals is a novel and robust detection method suitable for preliminary screening method for detecting heavy metals in agriculture and environment samples. Stem bromelain has been previously showed to be sensitive towards mercury and copper. However, commercial preparation is in crude form and contains possible interfering entities. Using ion exchange and ...

Long-term water contamination, including heavy metals, may harm the reputation of location. Because this, it is necessary to do regular quality checks. In light high expense instrument monitoring, biomonitoring based on enzymes rapidly gaining popularity around world. For purpose this investigation, a molybdenum-reducing enzyme from Serratia marcescens strain DRY6 that sensitive metals was used...

Near-real-ime assay is anassay method that the whole process from sampling until results could be obtained in approximately Iess than one hour. The ElIman assay for acetyl cholinesterase (AChE) has near real-time potential due to its simplicity and fast assay time. The commercial acetylcholinesterase from Electrophorus electricus is well known for its uses in insecticides detection. A lesser kn...

Interest in the biologic role of trace elements prompted an investigation of their renal excretion. Preliminary qualitative analysis revealed that except for cobalt the entire first transition series of metals, namely, titanium, vanadium, chromium, manganese, iron, nickel and copper, were detectable in most human urines. In addition the heavier transition metals, molybdenum and silver, as well ...

Fruits of Solanum Lycopersicum, well-known as tomatoes, have got an high contents of essential nutritive elements for human health. They are rich of vitamins C, A, B group, D, K and minerals (P, Fe, Ca, Mg, Mn, Cu, K, Na) besides to be very rich in flavonoids and lycopene (1, 2). These characteristics make tomatoes the best allies against cancer disease and an essential food for a good health. ...

The sensitizing properties of metals widely used in medical and dental care have been studied with the help of an optimized lymphocyte proliferative assay, MELISA. MELISA (memory lymphocyte immuno-stimulation assay) was originally developed for the screening of allergenic epitopes of drugs and other chemicals of low molecular weight, but has recently been adapted for the study of metal-induced ...

Siderophores are iron chelating substances that are also known to bind metals such as Molybdenum and Lead. The pigment produced by P. fluorescens is a siderophore whose ability to bind to different metal ions was investigated in this study. Pigment production was increased in the presence of Sodium, Potassium, Lead, Molybdenum and Cadmium. Siderophores were detected using CAS assay and catechol...