Metalloproteins are essential in biology. The incorporation of metal ion into metalloproteins significantly expands protein functionality and enhances protein stability. Over the last few years, atomic force microscopy-based single molecule force spectroscopy (SMFS) has evolved into a unique tool allowing for probing metalloproteins and metalligand bonds one molecule/bond at a time. Mechanical ...

Metalloproteins have many different functions in cells such as enzymes; signal transduction, transport and storage proteins. About one third of all proteins require metals to carry out their functions. In the present study we have analyzed the roles played by Arg and Lys (cationic side chains) interactions with π (Phe, Tyr or Trp) residues and their role in the structural stability of metallopr...

Metalloproteins are a class of biologically important macromolecules, which have various functions such as oxygen transport, electron transfer, oxidation, and oxygenation. These diverse functions of metalloproteins have been thought to depend on the ligands from amino acid, coordination structures, and protein structures in immediate vicinity of metal ions. In this project, we are studying the ...

Metalloproteins are a class of biologically important macromolecules, which have various functions such as oxygen transport, electron transfer, oxidation, and oxygenation. These diverse functions of metalloproteins have been thought to depend on the ligands from amino acid, coordination structures, and protein structures in immediate vicinity of metal ions. In this project, we are studying the ...

Metalloproteins (metal-binding proteins) refer to proteins containing metal ion cofactors. The importance of these proteins has increased owing to their involvement in many biological processes. Here, we introduce an analytical platform based on online coupling of miniaturized asymmetrical flow field-flow fractionation (mAF4) and inductively coupled plasma mass spectrometry (ICPMS) for size sep...

Metalloproteins are a class of biologically important macromolecules, which have various functions such as oxygen transport, electron transfer, oxidation, and oxygenation. These diverse functions of metalloproteins have been thought to depend on the ligands from amino acid, coordination structures, and protein structures in immediate vicinity of metal ions. In this project, we are studying the ...

Approximately one-third of all proteins have been estimated to contain at least one metal cofactor, and these proteins are referred to as metalloproteins. These represent one of the most diverse classes of proteins, containing metal ions that bind to specific sites to perform catalytic, regulatory and structural functions. Bioinformatic tools have been developed to predict metalloproteins encod...

Metalloproteins play indispensable roles in biology owing to the versatile chemical reactivity of metal centres. However, studying their reactivity in many metalloproteins is challenging, as protein three-dimensional structure encloses labile metal centres, thus limiting their access to reactants and impeding direct measurements. Here we demonstrate the use of single-molecule atomic force micro...

Metals are essential for protein function as cofactors to catalyze chemical reactions. Disruption of metal homeostasis is implicated in a number of diseases including Alzheimer's and Parkinson's disease, but the exact role these metals play is yet to be fully elucidated. Identification of metalloproteins encounters many challenges and difficulties. Here we report an approach that allows metallo...