نتایج جستجو برای: mediated isothermal amplification

تعداد نتایج: 472108  

Journal: :jundishapur journal of microbiology 0
somaye bakhtiari department of microbiology, school of medicine, kermanshah university of medical sciences, kermanshah, ir iran amirhooshang alvandi department of microbiology, school of medicine, kermanshah university of medical sciences, kermanshah, ir iran hamid pajavand department of microbiology, school of medicine, kermanshah university of medical sciences, kermanshah, ir iran jafar navabi imam khomeini hospital, kermanshah university of medical sciences, kermanshah, ir iran farid najafi department of biostatistics and epidemiology, school of hygiene, kermanshah university of medical sciences, kermanshah, ir iran ramin abiri department of microbiology, school of medicine, kermanshah university of medical sciences, kermanshah, ir iran; department of microbiology, school of medicine, kermanshah university of medical sciences, p. o. box: 6714869914, kermanshah, ir iran. tel: +98-9122773648, fax: +98-8314274623

conclusions the analytical sensitivity of lamp and pcr methods, using the designed primers, was 8 times more than any other reported methods. the designed methods are specific and sensitive for detection of h. pylori in different clinical and environmental samples. results the detection limits of the lamp and pcr assay were similar and were 10 fg of pure dna of h. pylori, which is equal to 6 co...

Journal: :iranian journal of microbiology 0
reza ranjbar molecular biology research center, baqiyatallah university of medical sciences, tehran, iran. davoud afshar molecular biology research center, baqiyatallah university of medical sciences, tehran, iran.

background and objectives: loop-mediated isothermal amplification is a novel nucleic acid amplification assay providing as a simple diagnostic tool for rapid identification of microbial diseases in developing countries. in this study, a lamp assay was established for yersinia enterocolitica , a leading cause of acute enterocolitis in young children. materials and methods: lamp assay was establi...

ژورنال: :دانش و تندرستی 0
massomeh masoomikarimi mehdi mirzaei pirasteh norouzi mojgan fazli sanaz dehbashi davoud afshar

مقدمه: استرپتوکوکوس پیوژنز کلونیزه کننده پوست و گلو در بسیاری از انسان ها است. این باکتری علاوه بر عفونت های چرکی حاد، مسئول عوارض و بیماری های خودایمن بعد از عفونت شامل آرتریت واکنشی، گلومرولونفریت و تب روماتیسمی می باشد. روش استاندارد طلایی برای شناسایی استرپتوکوکوس پیوژنز، کشت است. اما به دلیل زمان بر بودن روش مذکور و اهمیت تشخیص سریع آن، استفاده از روش های مولکولی مفید است. تکنیک lamp روش ن...

Journal: :iranian journal of virology 0
b pourhossein department of virology, faculty of medical sciences, tarbiat modares university, tehran, iran h soleimanjahi department of virology, faculty of medical sciences, tarbiat modares university, tehran, iran f behzadian molecular genetics department, research center for sciences and biotechnology, tehran, iran b khansarinejad department of virology, faculty of medical sciences, tarbiat modares university, tehran, iran

background and aims: considering difficulties in usual laboratory methods in detection of viral infections, improved dna-based diagnostic techniques are more reliable. loop mediated isothermal amplification method (lamp) is a nucleic acid amplification method that amplifies dna using six primers which has been developed to diagnose viruses as a rapid and high efficiency test. in this study, the...

2013
Mohammad Pourhassan-Moghaddam Mohammad Rahmati-Yamchi Abolfazl Akbarzadeh Hadis Daraee Kazem Nejati-Koshki Younes Hanifehpour Sang Woo Joo

Different immunoassay-based methods have been devised to detect protein targets. These methods have some challenges that make them inefficient for assaying ultra-low-amounted proteins. ELISA, iPCR, iRCA, and iNASBA are the common immunoassay-based methods of protein detection, each of which has specific and common technical challenges making it necessary to introduce a novel method in order to ...

Journal: :Chemical communications 2011
Cuiping Li Zhengping Li Hongxia Jia Jingli Yan

An ultrasensitive microRNA assay was developed with one-step loop-mediated isothermal amplification (LAMP) initiated by the target microRNA.

Journal: :Journal of clinical microbiology 2012
Nobuo Koizumi Chie Nakajima Tsunehito Harunari Tsutomu Tanikawa Toshihiro Tokiwa Eriko Uchimura Tokujiro Furuya Claro Niegos Mingala Marvin Ardeza Villanueva Makoto Ohnishi Yasuhiko Suzuki

We developed a new loop-mediated isothermal amplification (LAMP) method to detect rrs, a 16S rRNA gene of pathogenic Leptospira spp. in urine. The method enables detection of two leptospiral cells per reaction mixture following boiling of urine specimens. The sensitivity of this method is higher than that of culture or of flaB nested PCR.

Journal: :Nippon Shokuhin Kagaku Kogaku Kaishi 2014

کرمی, علی ,

مقدمه:سالمونلاها از مهمترین باکتری های بیماری و عامل تیفوئید، باکتریمی، آنتروکولیت و سالمونلوز است که از عفونت های مشترک انسان و حیوانات می باشد. بیماریهای ناشی از این عامل یک معضل بزرگ بهداشتی در جهان به خصوص در کشورهای در حال توسعه از جمله کشور ما می باشد. بنابراین تشخیص سریع، دقیق و به موقع آن برای جلوگیری از اپیدمی و عوارض مخرب این باکتری ضروری به نظر می رسد. برای تشخیص سالمونلا روش های مخت...

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