Gene therapy for congenital protein deficiencies requires lifelong expression of a deficient protein. Current gene therapy approaches preferentially employ the strong cytomegalovirus (CMV) promoter/enhancer or its derivative CAG promoter; however, these promoters provide only temporary transgene expression. To create a promoter that enables long-lasting expression in muscle, hybrid promoters we...

This study draws upon a research in investigating the enactment of primary teachers’ mathematics Content Knowledge (MCK) and Mathematics Pedagogical Content Knowledge (MPCK) in practice. The teaching on ratio and proportion of two teachers with different level MCK and MPCK were observed within video observation framework which involves qualitative research through case study. The result indicat...

Chemokine homologs are encoded by many large DNA viruses, suggesting that they contribute to control of host leukocyte transmigration and trafficking during viral infection. Murine cytomegalovirus carries a CC (beta) chemokine homolog gene giving rise to two related proteins, murine cytomegalovirus chemokine 1 and 2 (MCK-1 and MCK-2). MCK-1 peptide was found to induce calcium signaling and adhe...

To identify new gene regulatory pathways controlling skeletal muscle energy metabolism, comparative studies were conducted on muscle-specific transgenic mouse lines expressing the nuclear receptors peroxisome proliferator-activated receptor a (PPARa; muscle creatine kinase [MCK]-PPARa) or PPARb/d (MCK-PPARb/d). MCK-PPARb/d mice are known to have enhanced exercise performance, whereas MCK-PPARa ...

The second author’s name is spelled incorrectly. The correct name is: Lauren A. Howell. The correct citation is: Korrapati MC, Howell LA, Shaner BE, Megyesi JK, Siskind LJ, et al. (2013) Suramin: A Potential Therapy for Diabetic Nephropathy. PLoS ONE 8(9): e73655. doi:10.1371/journal.pone.0073655 The Author Contributions should read: Conceived and designed the experiments: MCK LJS RGS. Performe...

Lipoprotein lipase (LPL) has been implicated in the delivery of chylomicron-located alpha-tocopherol (alpha-TocH) to peripheral tissues. To investigate the role of LPL in the cellular uptake of alpha-TocH in peripheral tissue in vivo, three lines of transgenic mice [mouse creatine kinase- (MCK) L, MCK-M and MCK-H] expressing various amounts of human LPL were compared with regard to alpha-TocH l...

Lipoprotein lipase (LPL) is the rate-limiting enzyme for the import of triglyceride-derived fatty acids by muscle, for utilization, and adipose tissue (AT), for storage. Relative ratios of LPL expression in these two tissues have therefore been suggested to determine body mass composition as well as play a role in the initiation and/or development of obesity. To test this, LPL knockout mice wer...

Inclusion body myositis, the most common muscle disorder in the elderly, is partly characterized by abnormal expression of amyloid precursor protein (APP) and intracellular accumulation of its proteolytic fragments collectively known as β-amyloid. The present study examined the effects of β-amyloid accumulation on mitochondrial structure and function of skeletal muscle from transgenic mice (MCK...

We have studied the electrophoretic characteristics of a creatine kinase (CK, EC 2.7.3.2) isoenzyme, macro creatine kinase type 2 (MCK-2), in the serum of 42 patients with carcinoma of the colon. The patients could be divided into three subgroups by the intensity of the MCK-2 band (nine high, nine medium, and 24 low intensity), but we detected no correlation between the isoenzyme activity and t...

Sera from 30 patients, selected only on the basis of having serum theophylline concentrations greater than or equal to 18 mg/L, were assayed for total creatine kinase (CK), isoenzyme CK-MB, and cathodally migrating CK (mCK-2). CK-MB was measured by ion-exchange chromatography. mCK-2 was detected by electrophoresis on agarose gel, with fluorometric scanning. The mCK-2 variant was found in 37% of...