We previously reported a novel affinity purification (AP) method termed modified chromatin immunopurification (mChIP), which permits selective enrichment of DNA-bound proteins along with their associated protein network. In this study, we report a large-scale study of the protein network of 102 chromatin-related proteins from budding yeast that were analyzed by mChIP coupled to mass spectrometr...

BACKGROUND The MChip uses data from the hybridization of amplified viral RNA to 15 distinct oligonucleotides that target the influenza A matrix (M) gene segment. An artificial neural network (ANN) automates the interpretation of subtle differences in fluorescence intensity patterns from the microarray. The complete process from clinical specimen to identification including amplification of vira...

The performance of a diagnostic microarray (the MChip assay) for influenza was compared in a blind study to that of viral culture, reverse transcription (RT)-PCR, and the QuickVue Influenza A+B test. The patient sample data set was composed of 102 respiratory secretion specimens collected between 29 December 2005 and 2 February 2006 at Scott & White Hospital and Clinic in Temple, Texas. Samples...

Acknowledgments We would like to extend our sincere gratitude to all of those who helped us develop this framework. We would particularly like to thank the CORE group for allowing us to present an earlier version of the framework at their fall meetings in Washington, D.C. Tsuma and their colleagues at the Maternal and Child Health Integrated Program office in Washington, D.C., and from Nahed Ma...

We have proposed a very high speed internet (VHSI) abstraction that can provide a variable grade service with performance guarantees on top of diverse networks. An important component of the VHSI abstraction is a novel Multipoint Congram-oriented High Performance Internet Protocol (MCHIP). Features of this protocol include support for multipoint communication, the congram as the service primiti...

BACKGROUND Based on the established protective effect of voluntary medical male circumcision (VMMC) in reducing female-to-male HIV transmission, Tanzania's Ministry of Health and Social Welfare (MOHSW) embarked on the scale-up of VMMC services in 2009. The Maternal and Child Health Integrated Project (MCHIP) supported the MOHSW to roll out VMMC services in Iringa and Njombe, 2 regions of Tanzan...

Country and HQ Contacts Aissata (Aida) Lo, Anita Gibson, Pat Taylor, Rebecca Levine, Erin Fleming, Alyssa Om’Iniabohs, Winnie Mwebesa, Karen Waltensperger, Eric Swedberg, Serge Raharison, Nefra Faltas, Soo Kim, Holly Blanchard, Anne Pfitzer, Tsigue Pleah, Devon Mackenzie, Bethany Arnold, Ian Moise, Aimee Dickerson Selected Health and Demographic Data for Mali Maternal mortality ratio (deaths/10...

Currently, diagnostic testing for Lyme disease is done by determination of the serologic responses to Borrelia burgdorferi antigens, with the exception of the early localized phase of disease where diagnosis must be done clinically. Here, we describe the use of microfluidics technology to develop a multiplexed rapid lab-on-a-chip point of care (POC) assay for the serologic diagnosis of human Ly...

This Postpartum Hemorrhage: Prevention and Management toolkit was developed by the USAID-funded Maternal and Child Health Integrated Program (MCHIP) as a resource of current evidence, materials and experiences from around the world. It reflects contributions from many donors, agencies, associations, academic institutions and organizations which have identified postpartum hemorrhage as a priorit...