objective: n present study effect of various factors including sucrose, agar, peg, agno3, activated charcoal on somatic embryo induction and its subsequent regeneration was investigated using varieties dom siah and nemat. methods: 330 explant (mature embryos) of each varieties were cultured, 33/plate (100 x 15 mm), on callus induction murashige and skoog (ms) medium (murashige and skoog 1962) s...

BACKGROUND AND AIMS Sunflower cultivars exhibit a wide range of oil content in the mature achene, but the relationship between this and the dynamics of oil deposition in the achene during grain filling is not known. Information on the progress, during the whole achene growth period, of the formation of oil bodies in the components of the achene and its relationship with variations in final oil ...

embryo culture were used to produce inter specific walnut hybrid plants between juglans nigra l. and j. regia l. developed ovaries were collected from j. rejia female flowers which were pollinated with j. nigra pollen grain by high dusting on a mature tree. in order to isolate embryos, exocarps were aseptically removed. isolated embryos from ovules at different developmental stages were transfe...

abstract in this experiment the effects of two type of medium cultures, gibberellic acid and dark/cold and light/warm culture conditions on mature embryos germination of a local cultivar of walnut were investigated. the mature fruits were collected at 22 weeks postanthesis and after disinfection the mature embryo along with cotyledon pieces were excised and then were cultured on mt and modified...

The embryo sac, the female gametophyte of flowering plants, plays important roles in the pollination and fertilization process. Maize (Zea mays L.) is a model monocot, but little is known about the interactions between its embryo sac and the pollen tube. In this study, we compared the transcript profiles of mature embryo sacs, mature embryo sacs 14-16 h after pollination, and mature nucelli. Co...

The present study establishes a regeneration protocol and optimizes conditions for Agrobacterium-mediated transformation of the tetraploid emmer wheat, Triticum dicoccum. Regeneration from mature and immature embryos was accomplished as a two-step process involving callus induction in the presence of 2,4-D followed by regeneration on a 2,4-D free, cytokinin-containing medium (RM1). Higher conce...

Embryo culture were used to produce inter specific walnut hybrid plants between Juglans nigra L. and J. regia L. Developed ovaries were collected from J. rejia female flowers which were pollinated with J. nigra pollen grain by high dusting on a mature tree. In order to isolate embryos, exocarps were aseptically removed. Isolated embryos from ovules at different developmental stages were transfe...

The continuous growth of the plant embryo is interrupted during the seed maturation processes which results in a dormant seed. The embryo continues development after germination when it grows into a seedling. The embryo growth phase starts after morphogenesis and ends when the embryo fills the seed sac. Very little is known about the processes regulating this phase. We describe mutants that aff...