objective: development of high producing mammalian cell lines is a major bottleneck in manufacturing of recombinant therapeutic proteins. this study examines the effect of using the matrix attachment region from the human interferon beta gene in combination with promoter activation strategy with e1a 13s protein on human tissue plasminogen activator (t-pa) expression in chinese hamster ovary (ch...

The 5-upstream region of a winged bean chymotrypsin inhibitor gene (JVCI-3b) was found to have a high affinity for nuclear matrix. The region, named WCI-3b MAR (matrix attachment region), is highly A + T-rich and contains multiple sites interacting with nuclear matrix. A MAR was also found in the corresponding region of the WCI-x gene, another active gene of the WCI family. Several MAR-binding ...

We explored the role that thrombospondin (TSP). a multifunctional extracellular matrix protein, plays in hematopoietic cell-cell and cell-matrix interactions. Thrombospondin synthesis is differentially regulated in human long-term bone marrow cultures. Consistent with this, human hematopoietic progenitor cells of all three lineages (erythrocyte, megakaryocyte, and granulocyte) use TSP as an att...

Matrix attachment regions (MAR) generally act as epigenetic regulatory sequences that increase gene expression, and they were proposed to partition chromosomes into loop-forming domains. However, their molecular mode of action remains poorly understood. Here, we assessed the possible contribution of the AT-rich core and adjacent transcription factor binding motifs to the transcription augmentin...

In regions of strong sea surface temperature (SST) gradients, the surface "geostrophic" currents have a vertical shear aligned with the surface density front defined by the temperature. This surface geostrophic ("thermal wind") shear can balance a portion of the surface wind stress, altering the classic Ekman response to wind forcing. Here we show that these frontal effects cannot be ignored in...

Recognition of a person through his face is the primitive mean of human identification. Identifying a person through face biometric have grown its importance through the last decade and researchers have attempted to find unique facial feature-points. Facial data also contains change with expression and age, which makes recognition through face difficult. And there has developed a stringent nece...

The eukaryotic genome is partitioned into chromatin domains containing coding and intergenic regions. Insulators have been suggested to play a role in establishing and maintaining chromatin domains. Here we describe the identification and characterization of two separable enhancer blocking elements located in the 5' flanking region of the chicken alpha-globin domain, 11-16 kb upstream of the em...

The chromosome modal number in Muscoidea Diptera is 2n=12, including five pairs of autosomes and one sex chromosome pair. Nevertheless, some species with 2n=10 chromosomes have been described, all of them from the Muscidae family. We analyzed the karyotype of some Muscidae species from different subfamilies and compared the obtained data with the karyotypes of some species of the families Calli...