A multiplex PCR test for determining mating type of the pathogens Tapesia yallundae and Tapesia acuformis is described. The test involves three primers: a "common" primer annealing to DNA sequence conserved in the flanking region of both mating-type idiomorphs and two specific primers annealing to sequence in either the MAT-1 or the MAT-2 idiomorphs. Locating the specific primers in different p...

Homokaryons from the homothallic ascomycte Sordaria macrospora are able to enter the sexual pathway and to form fertile fruiting bodies. To analyze the molecular basis of homothallism and to elucidate the role of mating-products during fruiting body development, we cloned and sequenced the entire S. macrospora mating-type locus. Comparison of the Sordaria mating-type locus with mating-type idio...

to evaluate fertility status and mating type distribution of cryphonectria parasitica, causal agent of chestnut blight in guilan province, four sites in two main growing regions of: shaft (visrud, taleghan and babarekab), and rezvanshahr (doran) were investigated. during the study, fifty four isolates of c. parasitica were examined. c. parasitica isolates were crossed with each of the two matin...

fusarium wilt caused by fusarium oxysporum schltdl. f.sp. phaseoli (fop), is one of the important diseases of the common bean in iran and many of the bean growing countries. incidence of the disease has been reported in tropical and semi-tropical regions of the world. this study was carried out to characterize genetic diversity of f. oxysporum isolates from the common bean by vegetative compati...

In heterothallic Ascomycota, two opposite but distinct mating types control all sexual processes. Using mating crosses, mating types were assigned to ten isolates of the heterothallic fungal species Ophiostoma quercus. Primers were subsequently designed to target the MAT1-1-1, MAT1-1-3 (of the mating type 1 idiomorph), and MAT1-2-1 (of the mating type 2 idiomorph) genes in these isolates. Resul...

Leptographium procerum and the closely related species Leptographium profanum, are ascomycetes associated with root-infesting beetles on pines and hardwood trees, respectively. Both species occur in North America where they are apparently native. L. procerum has also been found in Europe, China New Zealand, and South Africa where it has most probably been introduced. As is true for many other L...

Two mating-type genes, designated MAT1-1-1 and MAT1-2-1, were cloned and sequenced from the presumed asexual ascomycete Cladosporium fulvum (syn. Passalora fulva). The encoded products are highly homologous to mating-type proteins from members of the Mycosphaerellaceae, such as Mycosphaerella graminicola and Cercospora beticola. In addition, the two MAT idiomorphs of C. fulvum showed regions of...

Sexual reproduction in filamentous ascomycetes is controlled by the mating type (MAT) locus, including two idiomorphs MAT1-1 and MAT1-2 Understanding the MAT locus can provide clues for unveiling the sexual development and virulence factors for fungal pathogens. The genus Valsa (Sordariomycetes, Diaporthales) contains many tree pathogens responsible for destructive canker diseases. The sexual s...

Fusarium wilt caused by Fusarium oxysporum Schltdl. f.sp. phaseoli (Fop), is one of the important diseases of the common bean in Iran and many of the bean growing countries. Incidence of the disease has been reported in tropical and semi-tropical regions of the world. This study was carried out to characterize genetic diversity of F. oxysporum isolates from the common bean by vegetative compati...