نتایج جستجو برای: mannanase

تعداد نتایج: 482  

2014
Yotthachai Piwpankaew Supa Sakulsirirat Sunee Nitisinprasert Thu-Ha Nguyen Dietmar Haltrich Suttipun Keawsompong

The mannanase gene of B. circulans NT 6.7 was cloned and expressed in Escerichia coli and food-grade Lactobacillus plantarum expression system. B. circulans NT 6.7 mannanase gene consisted of 1,083 nucleotides encoding 360 amino acid residues which belonging to glycosyl hydrolase family 26. In E. coli system, mannanase gene was cloned into pET21d and expressed in E. coli BL21* (DE3). Beta-manna...

Journal: :The Biochemical journal 1995
K L Braithwaite G W Black G P Hazlewood B R Ali H J Gilbert

Pseudomonas fluorescens subsp. cellulosa when cultured in the presence of carob galactomannan degraded the polysaccharide. To isolate gene(s) from P. fluorescens subsp. cellulosa encoding endo-beta-1,4-mannanase (mannanase) activity, a genomic library of Pseudomonas DNA, constructed in lambda ZAPII, was screened for mannanase-expressing clones using the dye-labelled substrate, azo-carob galacto...

2011
Pankaj Agrawal Dheeraj Verma Henry Daniell

Lignocellulosic ethanol offers a promising alternative to conventional fossil fuels. One among the major limitations in the lignocellulosic biomass hydrolysis is unavailability of efficient and environmentally biomass degrading technologies. Plant-based production of these enzymes on large scale offers a cost-effective solution. Cellulases, hemicellulases including mannanases and other accessor...

Journal: :Journal of biotechnology 2010
Tuan Anh Pham Jean Guy Berrin Eric Record Kim Anh To Jean-Claude Sigoillot

Endo beta-1,4-mannanases (beta-mannanases, EC 3.2.1.78), belonging to CAZy GH5 and GH26 families, catalyze the hydrolysis of structurally different mannans. In this study, the mannanase encoding gene of Aspergillus aculeatus VN was expressed in Aspergillus niger D15#26 using pAN 52-4 vector, under the control of PgpdA promoter and TtrpC terminator. In order to improve the hydrolytic capacity of...

2015
Ebaa Ebrahim El-Sharouny Nabil M.K. El-Toukhy Nermeen Ahmed El-Sersy Abeer Abd El-Aal El-Gayar

An alkaliphilic-thermotolerant Bacillus cereus N1 isolated from Bani Salama Lake, Wadi El-Natron, Egypt, was proved to produce mannanase enzyme. Optimization of the fermentation medium components using Plackett-Burman design was applied. Glucose and inoculum size were found to be the most significant factors enhancing the production of the enzyme. On applying optimized medium in the fermentatio...

2014
Nalan Ozgur Yiğit Seval Bahadir Koca Behire Isıl Didinen Ibrahim Diler

A 12-week feeding trial was conducted with 87 g rainbow trout to evaluate the effects on growth performances, feed efficiency and nutrient digestibility of adding β-mannanase and α-galactosidase enzymes, solely or in combination. Seven diets were prepared by adding β-mannanase, α-galactosidase and mixed enzyme at two different levels (1 g/kg and 2 g/kg) to control diet (without enzyme) includin...

Journal: :Acta crystallographica. Section F, Structural biology and crystallization communications 2012
Kimihiko Mizutani Sae Tsuchiya Mayuko Toyoda Yuko Nanbu Keiko Tominaga Keizo Yuasa Nobuyuki Takahashi Akihiko Tsuji Bunzo Mikami

β-1,4-Mannanase (EC 3.2.1.78) catalyzes the hydrolysis of β-1,4-glycosidic bonds within mannan, a major constituent group of the hemicelluloses. Bivalves and gastropods possess β-1,4-mannanase and may degrade mannan in seaweed and/or phytoplankton to obtain carbon and energy using the secreted enzymes in their digestive systems. In the present study, the crystal structure of AkMan, a gastropod ...

Journal: :Journal of animal science 2010
S Y Yoon Y X Yang P L Shinde J Y Choi J S Kim Y W Kim K Yun J K Jo J H Lee S J Ohh I K Kwon B J Chae

Four experiments were conducted to determine the effects of dietary supplementation of corn distillers dried grain with solubles (DDGS) diets with mannanase on performance, apparent total tract digestibility (ATTD) of energy and nutrients, blood metabolites, and carcass characteristics of grower-finisher pigs. In Exp. 1, 96 grower pigs (initial BW, 57.6 kg), 6 pigs per pen and 4 pens per treatm...

Journal: :Plant physiology 2000
H Nonogaki O H Gee K J Bradford

Endo-beta-mannanase (EC 3.2.1.78) is involved in hydrolysis of the mannan-rich cell walls of the tomato (Lycopersicon esculentum Mill.) endosperm during germination and post-germinative seedling growth. Different electrophoretic isoforms of endo-beta-mannanase are expressed sequentially in different parts of the endosperm, initially in the micropylar endosperm cap covering the radicle tip and s...

2014
Yotthachai Piwpankaew Supa Sakulsirirat Sunee Nitisinprasert Thu-Ha Nguyen Dietmar Haltrich Suttipun Keawsompong

The mannanase gene of B. circulans NT 6.7 was cloned and expressed in an Escherichia coli expression system. The B. circulans NT 6.7 mannanase gene consists of 1,083 nucleotides encoding a 360-amino acid residue long polypeptide, belonging to glycoside hydrolase family 26. The full-length mannanase gene including its native signal sequence was cloned into the vector pET21d and expressed in E. c...

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