Mistic, a bacterial membrane-associating protein family, uniquely found in Bacillus species. It enhances expression of eukaryotic membrane proteins at the bacterial membrane. Mistic from B. subtilis (M110), expresses at the Escherichia coli membrane, however its shorter orthologs have been recently shown to be mainly cytoplasmic with varying membrane affinities. Based on that, we hypothesized t...

Mistic represents a family of unique membrane-associating proteins originally found in Bacillus subtilis (M110). As a fusion partner, it has been shown to assist overexpression of foreign integral membrane proteins in E. coli. We have expressed shorter Mistic homologs from other Bacillus species and surprisingly, unlike M110, found them abundant in the cytoplasm. These Mistic homologs including...

Mistic represents a family of unique membrane-associating proteins originally found in Bacillus subtilis (M110). As a fusion partner, it has been shown to assist overexpression of foreign integral membrane proteins in E. coli. We have expressed shorter Mistic homologs from other Bacillus species and surprisingly, unlike M110, found them abundant in the cytoplasm. These Mistic homologs including...

Protein biosynthesis requires aminoacyl-transfer RNA (tRNA) synthetases to provide aminoacyl-tRNA substrates for the ribosome. Most bacteria and all archaea lack a glutaminyl-tRNA synthetase (GlnRS); instead, Gln-tRNA(Gln) is produced via an indirect pathway: a glutamyl-tRNA synthetase (GluRS) first attaches glutamate (Glu) to tRNA(Gln), and an amidotransferase converts Glu-tRNA(Gln) to Gln-tRN...

The biological activity of three monoclonal antibodies (mAbs) against the rabbit mammary prolactin (PRL) receptor (M110, A82, and A917) were investigated using explants of rabbit mammary gland. The three mAbs which were all able to inhibit the binding of 125I-ovine prolactin to its receptor had different biological activities. Two mAbs (M110 and A82) were able to prevent the stimulating effect ...

A proof of principle experiment is underway at the UCLA Neptune laboratory to test the concept of generating linearly ramped relativistic electron bunches (rising in density from head to tail followed by a sharp cutoff) by using a sextupole-corrected dogleg section as a bunch compressor. Bunches with this structure have been predicted to be ideal for use as a plasma wakefield drive beam. The di...

We present results from NICMOS Hubble Space Telescope observations of the reddened bulge globular clusters NGC 6528, Terzan 5, Liller 1, UKS 1 and Terzan 4, obtained through the filters F110W (1.1 μm) and F160W (1.6 μm) (nearly equivalent to J and H). For the first time the turnoff region of Liller 1 and the main sequence of Terzan 5 and Terzan 4 are reached, as well as the horizontal branch of...

Bananas are important crops in developing countries with tropical climate. In Mexico, the banana production has increased, and it must be guaranteed. The Panama disease, caused by fungus Fusarium oxysporum f.sp. cubense threatens current production, for what is necessary to implement methods protect this crop. Fungi from genus Trichoderma natural residents of rhizosphere. This comprises mycopar...