Lysolecithin comprises 9.6 and 21.5% of the phospholipids of the plasma of man and rat, respectively. Ultracentrifugal and gel filtration studies showed that the major portion of the lysolecithin is not found together with the other phospholipids in the plasma lipoproteins. By zone electrophoresis, gel filtration, and ammonium sulfate fractionation, it was found that lysolecithin was consistent...

The binding of apoA-I to lysolecithin has been studied by fluorescence and circular dichroism. The influence of the conformation of apoA-I on its interaction with lysolecithin has also been evaluated. ApoA-I is bound to lysolecithin with an association greater than 10(7) whether apoA-I is native or highly unfolded in 1.8 M guanidinium hydrochloride. The association of apoA-I with lysolecithin r...

We have studied the effect of long-term hyperlipemia and atherosclerosis in squirrel monkeys on the metabolism of lysolecithin-(14)C (1-palmitoyl-1'-(14)C sn-glycerol 3-phosphorylcholine) in order to explain elevated plasma and arterial concentrations of lysolecithin. The die-away curves of lysolecithin-(14)C from plasma and the timing of appearances of other (14)C-labeled moieties in plasma an...

Human platelets incubated with 32p lysolecithin convert this compound to platelet lecithin and the water-soluble product glycerylphosphorylcholine. Lecithin synthesis from lysolecithin by intact platelets has a pH optimum of 9, whereas platelet homogenates show a pH optimum of 7.5 for the same reaction. Conversion of lysolecithin to platelet lecithin takes place by direct acylation of lysolecit...

Hen erythrocytes that were fixed after treatment with lysolecithin in aqueous solution for 30 s at 37 °C showed evidence of bridge formation between adjacent lysed cells. Generally, the homokaryons that were produced using lysolecithin in this way contained large numbers of nuclei. These giant syncytia had damaged nuclear membranes and unstable plasma membranes; complete disintegration of the s...

Rabbit polymorphonuclear leukocytes ingesting paraffin oil particles stabilized with albumin, converted more lysolecithin-(32)P (added to the medium as an albumin complex) to cellular lecithin than did control cells. Almost all of the increment in leukocyte lecithin-(32)P is found in association with the isolated phagocytic vacuoles. About half of lecithin-(32)P of granulocytes incubated first ...

Lysolecithin is preferentially used in food technology, in animal feed products, and in cosmetics and pharmaceutical preparations as a surfactant.i} Industrially, lysolecithin s produced by enzymatic hydrolysis of Iecithin, The most eommon enzyme used is pancreatin prepared from porcine pancreas. However, the use of pancreatin has certain disadvantages, such as its limited supply, its high cost...

The effects of lysolecithin and hexadecyltrimethylammonium bromide on the structure and stability of apoA-II from human high density lipoprotein have been evalued by circular dichroism and fluorescence measurements. There is a profound enhancement in the stability of apoA-II to guanidinium hydrochloride denaturation when it forms a phospholipid complex with lysolecithin micelles. This complex i...