background: sertoli cells play a pivotal role in creating microenvironments essential for spermatogonial stem cells (sscs) self-renewal and commitment to differentiation. maintenance of sscs and or induction of in vitro spermiogenesis may provide a therapeutic strategy to treat male infertility. objective: this study investigated the role of luekemia inhibitory factor (lif) on the propagation o...

BACKGROUND Sertoli cells play a pivotal role in creating microenvironments essential for spermatogonial stem cells (SSCs) self-renewal and commitment to differentiation. Maintenance of SSCs and or induction of in vitro spermiogenesis may provide a therapeutic strategy to treat male infertility. OBJECTIVE This study investigated the role of luekemia inhibitory factor (LIF) on the propagation o...

background: leukemia inhibitory factor (lif) is a neurortophic cytokine which plays an important role in the neural cell survival. expression of lif and its receptor, lifr, in different brain regions has been demonstrated. based on evidences lif plays an important role in the modulation of neurogenesis and glial responses to injury. up-regulation of lif after central nervous system (cns) damage...

background: embryonic stem cells (esc) are pluripotent cells which have been used as a model to study the mechanism that control the embryogenesis and early mammalian development in vitro. the aim of this study was to isolate and produce embryonic stem cells from late blastocyst stage embryos in mice. materials and methods: blastocyst stage embryos from pregnant nmri mice were obtained and cult...

background: leukemia inhibitory factor (lif) is a 45-56 kda glycoprotein that has an important role in proliferation and embryo implantation. its effect on oocyte maturation and how to exert the function remained to be elucidated. methods: immature mice superovulated with human menopausal gonadotropin and germinal vesicle (gv) oocytes were obtained from ovary 48 hours after. gv oocytes were cul...

background: in acute myeloblastic leukemia, a large number of tumor suppressor genes are silenced through dna methylation such as cdkn2b & p73. wnt inhibitory factor 1 (wif1) and dickkopf-3 (dkk-1) are negative regulators of wnt signaling pathway. in the present study, we evaluated the methylation status of wif1 and dkk-1 genes in acute myeloblastic leukemia patients. patients and methods: bloo...

abstract  background: despite the long-standing association of macrophage migration inhibitory  factor (mif) with delayed-type hypersensitivity response, the potential role  of mif in chronic allograft nephropathy is unknown. the association between upregulation of mif expression, macrophage and t cell infiltration and the severity of  chronic allograft nephropathy suggests that mif may be an i...

background: as condition and component of culture determine fate map of spermatogonial stem cells (sscs), the aim of this study was to evaluate of growth factors gdnf, lif and ra on proliferation and differentiation of ssc. materials and methods: sscs were cultured in two groups: the first group gdnf and lif and the second group ra. the number of clumps and colony formation was monitored during...

background: leukemia inhibitory factor (lif) is a pleiotropic cytokine of interleukin-6 family with a remarkable range of biological actions such as proliferative effects on the granulosa and theca cells. the aim of this study was to evaluate the effects of lif on the growth and maturation of mouse fresh and vitrified preantral follicles, an in vitro model was developed. methods: the ovaries of...

background: hoxa11 and leukemia inhibitory factor (lif) and basic transcriptional element binding protein1 (bteb1) are expressed in endometrium throughout the menstrual cycle and show a dramatic increase during the mid-luteal phase at the time of implantation. in this case-control study, the expression pattern of these mrna was evaluated in patients with endometriosis at the time of implantatio...