case presentation we herein describe a case of a 7 year-old child affected by an overlap syndrome between type 2 autoimmune hepatitis and small duct primary sclerosing cholangitis. although characterized by a severe onset, the disease showed a good response to treatment with prednisone and azathioprine. conclusions the association of type 2 autoimmune hepatitis and small duct primary cholangiti...

Children with autoimmune chronic active hepatitis may have high titers of antibodies detected by immunofluorescence staining of hepatocytes and tubular cells in rat liver and kidney sections, respectively. These antibodies are directed against antigens contained in microsomal fractions prepared from these two organs. We have found that sera from these patients recognized a 50,000 mol wt protein...

Thyroid microsomal antigen was studied using sera from rats immunized with solubilized human thyroid microsomal fraction. The methods used were gel immunodiffusion and the fluorescent antibody technique (FAT). Kidney, liver and thyroid microsomal fractions were solubilized by 0.5% Triton X-100 PBS. Rat sera immunized with solubilized thyroid microsomal fraction had a few precipitin antibodies t...

Children with autoimmune hepatitis have high serum titers of antibodies directed against a 50-kD protein of rat liver endoplasmic reticulum. Affinity-purified anti-50-kD antibodies were used to screen a rat liver cDNA library in lambda GT-11 expression vector. 12 immunopositive clones were obtained. Crossreactivities between fusion proteins of these clones and the 50-kD protein was demonstrated...