نتایج جستجو برای: livak method
تعداد نتایج: 1630094 فیلتر نتایج به سال:
The aim of this study was to assess the expression of genes associated with cell stress response in the patient with chronic stuttering. We examined genes expression in a 26-year-old patient (D.K.) with chronic perseverative stuttering (CPS) syndrome, the symptoms of which intensified after repeated failure to find a job and break-up with his fiancée. The symptoms of stuttering were tested usin...
1.Battaglia, M., P. Pedrazzoli, B. Palermo, A. Lanza, F. Bertolini, N. Gibelli, G.A. Da Prada, A. Zambelli et al. 1998. Epithelial tumour cell detection and the unsolved problems of nested RT-PCR: a new sensitive one step method without false positive results. Bone Marrow Transplant 22:693-698. 2.Collins, C., J.M. Rommens, D. Kowbel, T. Godfrey, M. Tanner, S.I. Hwang, D. Polikoff, G. Nonet et a...
Latar Belakang: Kanker payudara merupakan jenis kanker dengan angka kejadiannya menempati posisi pertama terbanyak di Indonesia dibandingkan lain. Daun sirsak yang mengandung acetogenin memiliki efek sitotoksik terhadap sel T47D.
 Objektif: Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak daun level ekspresi gen p53 T47D secara in vitro.
 Metode: penelitian eksperimental met...
Leukemia 2001;15:1033–7. 14. Higuchi R, Fockler C, Dollinger G, Watson R. Kinetic PCR analysis: real-time monitoring of DNA amplification reactions. Biotechnology 1993;11:1026– 30. 15. Heid CA, Stevens J, Livak KJ, Williams PM. Real time quantitative PCR. Genome Res 1996;6:986–94. 16. Soong R, Beyser K, Basten O, Kalbe A, Rueschoff J, Tabiti K. Quantitative reverse transcription-polymerase chai...
1 Univ. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, Slovenia and rapid quantification results (Pfaffl, 2001; Yuan et al., 2006). Because of the lacking consensus on how to best perform qPCR, MIQE guidelines have been developed to uniform qPCR experiment setup, optimization and data analysis, making the protocols comparable between different research gro...
Citation: Ramalingam N, Fowler B, Szpankowski L, Leyrat AA, Hukari K, Maung MT, Yorza W, Norris M, Cesar C, Shuga J, Gonzales ML, Sanada CD, Wang X, Yeung R, Hwang W, Axsom J, Devaraju NSGK, Angeles ND, Greene C, Zhou M-F, Ong E-S, Poh C-C, Lam M, Choi H, Htoo Z, Lee L, Chin C-S, Shen Z-W, Lu CT, Holcomb I, Ooi A, Stolarczyk C, Shuga T, Livak KJ, Unger M and West JAA (2016) Fluidic Logic Used i...
The applications of conventional culture-dependent assays to quantify bacteria populations are limited by their dependence on the inconsistent success of the different culture-steps involved. In addition, some bacteria can be pathogenic or a source of endotoxins and pose a health risk to the researchers. Bacterial quantification based on the real-time PCR method can overcome the above-mentioned...
Anticancer Res 2003;23:3327–31. 4. Weikert S, Christoph F, Schrader M, Krause H, Miller K, Muller M. Quantitative analysis of survivin mRNA expression in urine and tumor tissue of bladder cancer patients and its potential relevance for disease detection and prognosis. Int J Cancer 2005;116:100–4. 5. Schultz IJ, Kiemeney LA, Karthaus HF, Witjes JA, Willems JL, Swinkels DW, et al. Survivin mRNA c...
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