Methods Stem Br (Vital Nutrients lot # 2890; Middletown, CT), was tested for authenticity and purity. Female C57BL/6J mice, (Jackson Laboratory; Bar Harbor, ME) received one bolus, i.p. injection of Br (12mg/kg) in 0.5ml of physiological saline and plasma collected at baseline 3, 6, 12 and 24 hours. All procedures were approved by the Animal Care Committee at University of CT Health Center. Pos...

The aim of this study was the development and validation of a new method for carbohydrates analyses obtained from woody biomass, as a step of bioethanol production. In this study, we have quantified carbohydrates in wood by gas chromatography (GC) after derivatization. Quantification of carbohydrates was made by liquid-liquid extraction, oximation and silylation and, finally, analysis by gas ch...

water contamination due to the wide variety of pesticides used in agriculture is a global environmental pollution problem. in order to reach at sub-î¼gl-1 levels of detection, an efficient extraction technique is required. a simple, fast and economical method, dispersive liquid-liquid micro extraction (dllme), followed by gas chromatography-mass spectrometry was assessed for determining endosul...

A validated method for the quantitative determination of venlafaxine, O-desmethylvenlafaxine in human plasma by tandem liquid chromatographymass spectrometry (HPLC/MS/MS) has been proposed. Sample preparation was carried out liquid-phase extraction with support (SLE, from «supported extraction») using HyperSEP cartridges and washing 3-methylbutyl ether. The measure target analytes on a triple q...

The conversion of deuterium-labeled ceramide to cerebroside has been studied. A mixture of N-(2’+hydroxy [4’,4’,5’,5’-2H4]hexadecanoyl) D-eryfhYO-1,3-dihydroxy-2amino-4-frans-[4,5-*H2]octadecene and N-(2’-D-hydroxy hexadecanoyl) D-eryfhro-l,3-dihydroxy-2-amino-4-frans-[33Hl]octadecene was incubated with mouse brain microsomes and a UDP-galactose regenerating system. Both galactosyl ceramide and...

a simple and rapid microextraction procedure based on dispersive liquid-liquid microextraction (dllme) was developed for determination and speciation of trace amounts of chromium (cr) by flame atomic absorption spectrometry (faas). in the proposed approach, chloroform and methanol were used as extraction and dispersive solvents. a new schiff's base ligand, bis(2-methoxy benzaldehyde) ethylene d...

Primary carnitine deficiency (PCD) is a rare autosomal recessive disorder, caused by deficiency in the plasma membrane carnitine transporter. In this report, we aimed to describe the diagnosis and treatment of 12 PCD patients. Blood spots were collected from the children, and analysed using liquid chromatography-mass spectrometry (LC-MS). Newborns and children who had repeated lower dried blood...