lipases are versatile biocatalysts with a wide range of application in food, dairy, leather, paper, pharmaceutical and detergent industries.  in this study, mesophilic lipase gene from an indigenous bacillus pumilus f3 that already its gene had been sequenced and  identified, was cloned and expressed in methylotrophic yeast pichia pastoris and codon translation of lipase gene was evaluated in p...

the present study was aimed to isolate and characterize the lipolytic enzyme producing bacteria from soil samples of regions around zayande-rood river of isfahan, iran. soil samples were collected from 15 cm depth of soil surface. based on morphology, distinct colonies were isolated and purified through streak culture on to standard agar plates. isolated colonies were examined for lipase activi...

the present study was undertaken to screen the soil samples collected in iran for the presence of the bacillus subtilis lipase a gene. the bacterial colonies obtained from the collected soil samples were examined by physical appearance, biochemical tests and  the polymerase chain reaction (pcr). only four colonies were identified as putative b. subtilis strains and all contained the lipase a ge...

The present study was undertaken to screen the soil samples collected in Iran for the presence of the Bacillus subtilis lipase A gene. The bacterial colonies obtained from the collected soil samples were examined by physical appearance, biochemical tests and  the polymerase chain reaction (PCR). Only four colonies were identified as putative B. subtilis strains and all contained the lipase A ge...

Background: Lipase enzymes are of great importance in various industries. Currently, extensive efforts have been focused on exploring new lipase producer microorganism as well as genetic and protein engineering of available lipases to improve their functional features. Methods: For screening lipase-producing lactobacilli, isolated strains were inoculated onto tributyrin agar plates. Molecular ...

Cloning and sequencing of a lipase gene from an indigenous Bacillus pumilus, strain F3, revealed an open-reading frame of 648 nucleotides predicted to encode a protein of 215 residues. Sequence analysis showed that F3 lipase contained a signal peptide composed of 34 amino acids with an H domain of 18 residues. A tat-like motif was found in the signal peptide similar to some other Bacillus pumil...

cloning and sequencing of a lipase gene from an indigenous bacillus pumilus, strain f3, revealed an open-reading frame of 648 nucleotides predicted to encode a protein of 215 residues. sequence analysis showed that f3 lipase contained a signal peptide composed of 34 amino acids with an h domain of 18 residues. a tat-like motif was found in the signal peptide similar to some other bacillus pumil...

background and objectives: lipases are particularly important due to the fact that they specifically hydrolyze acyl glycerol, oils and greases, which is of great interest for different industrial applications. materialst and methods : in this study, several lipase-producing bacteria were isolated from wastewater of an oil processing plant. the strain possessing the highest lipase activity was i...

using enrichment procedures, 45 organic solvent-tolerant lipase producer bacterial strains were screened from areas contaminated by oil and organic solvents. among the strains, 15 isolates exhibited extreme stability toward organic solvents and high lipolytic activity. the neb-1 isolate which was later identified as   pseudomonas sp. strain neb-1 by biochemical tests and 16s rdna gene sequ...