Enzyme cost is the major problem for industrial scale application. Immobilization is a promising approach to moderate the enzyme cost factor and increase its stability and activity. In this study, sol-gel method was used to prepare the immobilization platform and entrapped lipase as one of the most used enzyme in dairy processing, cosmetics and pharmaceutical industries. Lipase from Candida rug...

aquatic animals are important resources for extraction of valuable compounds such as enzymes. in this study, lipase was extracted from the intestine of rainbow trout. samples were defatted with acetone several times; ammonium sulphate (60%) was used for precipitation followed by ultrafiltration for concentrating the samples. the final purification of the enzyme was performed by passing of the s...

background and objectives: lipases are particularly important due to the fact that they specifically hydrolyze acyl glycerol, oils and greases, which is of great interest for different industrial applications. materialst and methods : in this study, several lipase-producing bacteria were isolated from wastewater of an oil processing plant. the strain possessing the highest lipase activity was i...

Pseudomonas aeruginosa BBRC-10036 was used for lipase production. The organism secreted the enzyme extracellulary. In order to purify the enzyme, precipitation was done first, and then this lipase has been purified by Ion exchange Chromatography leading to 2.3-fold purification and 11.47% recovery. Lipase from P.aeruginosa was entrapped into Ca-alginate gel beads and effect of independent varia...

background: nowadays it is proofed that the uterine artery plays essential role in follicular growth and/or post parturition hemorrhagic. objective: this study was conducted to evaluate the effect of bilateral uterine artery ligation (bual) on follicular fate and the probable histochemical changes of the carbohydrate and lipids in the ovaries of rabbits. materials and methods: 24 mature female ...

the tremendous mounting interest in lipase production is pertinent to the biotechnological applications that these enzymes offer. lipase, belonging to the hydrolyze enzymes, is involved in copious biological functions including: hydrolysis insoluble triacylglycerol to mono and diacylglycerols and glycerol. submerge fermentation technique was applied for the efficient production of this. briefly...

background: lipase is an enzyme with immense application potential. ester synthesis by lipase catalysis in organic media is an area of key industrial relevance. enzymatic preparations with traits that cater to the needs of this function are hence being intensely researched. objective: the objectives of the study were to immobilize the lipase from bacillus sp. ps35 by cross-linking and adsorptio...

the use of proteins and peptides as human therapeutics has been increased in recent years. lipase is a relatively homogeneous proteinaceous enzyme indicated in maldigestion. the purpose of the present study was to evaluate the effect of humidity along with compactional pressure on the enzymatic activity of wheat germ lipase. samples of lipase powder were kept at different relative humidity (rh)...

Enzyme efficiency was investigated in the lipase-catalyzed hydrolysis of canola oil in supercritical carbon dioxide (SCCO2). Immobilized lipase from Mucor miehie (Lipozyme IM) was used as the catalyst and the results showed that enzyme efficiency dropped at high pressures indicating a possible change in enzyme microstructure. Therefore, scanning electron microscopy (SEM) was used...