To the Editor: Several assays are available to measure human ghrelin (h-GHS), but the comparability of results among assays is unknown. As an addition to previous investigations concerning the stability of h-GHS (1 ), it was our objective to compare the two most commonly used commercial h-GHS RIAs, focusing on side-by-side determinations, recoveries, and assay imprecision. The assays were purch...

BACKGROUND The complex pathology of disease has sparked the development of novel protein expression profiling techniques that require validation in clinical settings. This study focuses on multiplexed analyses of adipocytokines and biomarkers linked to the metabolic syndrome, diabetes, and cardiovascular disease. METHODS Multiplexed immunoassays using fluorescent microspheres and the Luminex-...

The recent introduction of fluorescent bead-based technology, allowing the measurement of multiples analytes in a single 25-50 microl sample has revolutionized the study of cytokine responses. However, such multiplex approaches may compromise the ability of these assays to accurately measure actual cytokine levels. This study evaluates the performance of three commercially available multiplex c...

Nowadays antibiotics are used on a large scale in veterinary and human medicine to cure or prevent diseases. Some antibiotics injected into hatching eggs to eliminate pathogens and prevention of egg transmission of disease. Adverse effects of drugs have always been a major concern. There is scantly information available about the safety and pathological alterations of lincosamide-aminocyclitol ...

nowadays antibiotics are used on a large scale in veterinary and human medicine to cure or prevent diseases. some antibiotics injected into hatching eggs to eliminate pathogens and prevention of egg transmission of disease. adverse effects of drugs have always been a major concern. there is scantly information available about the safety and pathological alterations of lincosamide-aminocyclitol ...

Nowadays antibiotics are used on a large scale in veterinary and human medicine to cure or prevent diseases. Some antibiotics injected into hatching eggs to eliminate pathogens and prevention of egg transmission of disease. Adverse effects of drugs have always been a major concern. There is scantly information available about the safety and pathological alterations of lincosamide-aminocyclitol ...

Recent data suggest that proinsulin is associated with cardiovascular risk factors in nondiabetic and diabetic subjects. Since most conventional insulin assays cross-react with proinsulin, it has been suggested that the associations of insulin concentrations with dyslipidemia and hypertension could actually reflect associations with proinsulin. We examined these associations by using both a con...

Santica Marcovina, Ronald R. Bowsher, Greg Miller, Myrlene Staten, Gary Myers, Samuel P. Caudill, Scott E. Campbell, Michael W. Steffes for the Insulin Standardization Work Group. 1 Northwest Lipid Metabolism and Diabetes Research Laboratory, University of Washington, Seattle, WA 2 LINCO Diagnostic Services, St. Charles MO; B2S Consulting, Beech Grove, IN 3 Department of Pathology, Virginia Com...

Correction After publication of this work [1], it came to our attention that the laboratory we use changed the method of analysis for insulin and C-peptide during our data collection. Before March 2007 the method used for insulin was competitive radioimmunoassay (Linco Research Inc, St Charles, MO, USA) and for C-peptide competitive luminoimmunoassay (Immulite 2000, Diagnostic Products Corporat...

OBJECTIVE Adiponectin, an adipocyte-secreted hormone, circulates in the serum in several multimeric forms. Compared with total adiponectin, high-molecular weight (HMW) adiponectin has been suggested to be a better predictor of metabolic parameters and insulin sensitivity in humans. Our objective was to compare total adiponectin with HMW adiponectin as predictors of metabolic variables and insul...