نتایج جستجو برای: limiting dilution assay

تعداد نتایج: 322031  

Journal: :iranian journal of parasitology 0
somayeh ghotloo dept. of immunology, faculty of medicine, shahid beheshti university of medical sciences, tehran, iran mostafa haji mollahoseini dept. of immunology, faculty of medicine, shahid beheshti university of medical sciences, tehran, iran and dept of applied cell sciences, school of advance technologies in medicine, shahid beheshti university of medical sciences, tehran, iran ali najafi dept. of immunology, pasteur institute of iran, tehran, iran farshid yeganeh dept. of immunology, pasteur institute of iran, tehran, iran

background: limiting dilution assay is considered as the gold standard method for quantifying the number of parasites in the animal model of leishmania infection. nowadays, real-time pcr is being increasingly applied to quantify infectious agents. in the present study , a real-time pcr assay was developed to estimate para­site burdens in lymph nodes of leishmania major infected balb/c mice. enu...

2015
Somayeh GHOTLOO Mostafa HAJI MOLLAHOSEINI Ali NAJAFI Farshid YEGANEH

BACKGROUND Limiting dilution assay is considered as the gold standard method for quantifying the number of parasites in the animal model of Leishmania infection. Nowadays, real-time PCR is being increasingly applied to quantify infectious agents. In the present study, a real-time PCR assay was developed to estimate parasite burdens in lymph nodes of Leishmania major infected BALB/C mice. Enumer...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه پیام نور - دانشگاه پیام نور استان تهران - دانشکده علوم پایه 1388

بسمه تعالی چکیده به بیماری های حاصل از انگل های جنس لیشمانیا، لیشمانیوزیس گفته می شود. انگل پروتوزوای جنس لیشمانیا یک پاتوژن داخل سلولی است که می تواند باعث طیف وسیعی از بیماریهای انسانی از یک زخم پوستی تا عفونتهای احشایی را سبب شود. که با گزش پشه خاکی آلوده، به میزبان پستاندار از جمله انسان منتقل می شود. این بیماری در ?? کشور در مناطق گرمسیری و نیمه گرمسیری جهان گسترش یافته است و هم این...

Journal: :Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 1999
O L Caballero M C Costa A Trevisan R M Oliveira E A Viotti E R Távora S S Vilaça E Sabagga F J De-Paula P F Távora G Brasileiro-Filho L L Villa A J Simpson

To assess the clinical relevance of a semi-quantitative measurement of human cytomegalovirus (HCMV) DNA in renal transplant recipients within the typical clinical context of a developing country where virtually 100% of both receptors and donors are seropositive for this virus, we have undertaken HCMV DNA quantification using a simple, semi-quantitative, limiting dilution polymerase chain reacti...

Journal: :Journal of immunology 2001
T Bonnefoix P Bonnefoix M Callanan P Verdiel J J Sotto

Standardized statistical and graphical methods for analysis of limiting dilution assays are highly desirable to enable investigators to compare and interpret results and conclusions with greater accuracy and precision. According to these requirements, we present in this work a powerful statistical slope test that estimates the fit of the single-hit Poisson model to limiting dilution experiments...

Journal: :Journal of Clinical Microbiology 1994

Journal: :iranian journal of parasitology 0
t mohammadzadeh department of parasitology and mycology, school of medicine, shiraz university of medical sciences, shiraz, iran sm sadjjadi department of parasitology and mycology, school of medicine, shiraz university of medical sciences, shiraz, iran p habibi department of parasitology and mycology, school of medicine, shiraz university of medical sciences, shiraz, iran b sarkari basic in infectious diseases research center, shiraz university of medical sciences, shiraz, iran

background: leishmaniases are a group of diseases caused by leishmania parasites .  growing of drug unresponsiveness in leishmaniasis patients necessitates the development of new drugs and accor­dingly a suitable assay is needed for evaluation of any modalities.  the aim of this study was to compare four drug assays methods, agar dilution, broth dilution, cylinder plate and disk diffusion, for ...

2015
Daniel I. S. Rosenbloom Oliver Elliott Alison L. Hill Timothy J. Henrich Janet M. Siliciano Robert F. Siliciano

Limiting dilution assays are widely used in infectious disease research. These assays are crucial for current human immunodeficiency virus (HIV)-1 cure research in particular. In this study, we offer new tools to help investigators design and analyze dilution assays based on their specific research needs. Limiting dilution assays are commonly used to measure the extent of infection, and in the ...

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