purpose: to report the outcomes of conjunctival-limbal autograft (clau) in patients with unilateral total limbal stem cell deficiency (lscd) emphasizing surgical problems, complications, and their management. methods: in this prospective interventional case series, clau combined with amniotic membrane transplantation as a graft was performed on 26 patients with unilateral total lscd due to chem...

we are describing a new surgical approach in the management of advanced ocular surface cicatrization through a single procedure conjunctival-limbal-corneal allograft in which the external eye is resurfaced it involves a lamellar corneolimbal keratoplasty, 360° limbal stem cell transfer (on a “bipedicle” conjunctival-corneal carrier), and conjunctival tissue transplantation - all in a single con...

purpose: to evaluate stem cell deficiency using impression cytology (ic) in patients with chronic and delayed-onset mustard gas keratopathy (mgk). methods: a consecutive series of patients with mgk underwent ic. thirty-five eyes of 18 patients (all male) with mustard gas keratopathy were included in this observational case series. presence of goblet cells on the corneal side of specimens was co...

purpose: to describe a novel surgical technique for in vivo limbal stem cell cultivation for reconstruction of corneal epithelium in total limbal stem cell deficiency (lscd). methods: patients suffering from chronic total unilateral lscd due to a chemical injury were sequentially included. one small limbal block (2x1 mm) harvested from contralateral healthy eye sutured over the amniotic membran...

stem cells located at the limbus ,  are the ultimate source for regeneration of the corneal epithelium in the normal and traumatized states.  when limbal stem cells are dysfunctional or deficient, limbal stem cell deficiency (lscd) develops. its surgical management depends on laterality and severity of corneal-limbal involvement. conventional methods of stem cell transplantation are conjunctiva...

purpose: to evaluate the results of one 60-degree block conjunctival limbal autograft (clau) surgery in patients with unilateral total limbal stem cell deficiency (lscd). methods: five eyes of 5 patients were included. all patients had a history of chronic total unilateral lscd due to a chemical injury. two patients underwent one 60-degree block clau with amniotic membrane transplantation, two ...

purpose: to investigate the outcomes of keratolimbal allograft (klal) for treatment of total limbal stem cell deficiency (lscd). method: patients with total lscd and adequate tear production were included. a total of 27 klal procedures were performed in 21 eyes of 20 patients with lscd. immunosuppression was achived using mycophenolate and cyclosporine. main outcome measure was improvement in v...

purpose: to report the early results of transplantation of autologous limbal stem cell cultivated on amniotic membrane in total limbal stem cell deficiency. methods: four eye of 4 patients with total unilateral stem cell deficiency secondary to severe chemical or thermal burn were included. stem cell deficiency was confirmed with impression cytology in all cases. under topical anesthesia, a sma...

PURPOSE Limbal fibroblasts are known to regulate the maintenance and differentiation of the corneal epithelium including the limbal epithelial stem cells. This study examined the effect of limbal fibroblast conditioned media in a mouse model of limbal stem cell deficiency. METHODS Limbal stem cell deficiency was created in C57/Bl6 mice by performing a limbus to limbus epithelial debridement. ...

The cornea is the outermost tissue of the eye and it must be transparent for the maintenance of good visual function. The superficial epithelium of the cornea, which is renewed continuously by corneal stem cells, plays a critical role in the permanence of this transparency. These stem cells are localized at the cornea-conjunctival transition zone, referred to as the limbus. When this zone is af...