نتایج جستجو برای: light cycler qpcr

تعداد نتایج: 410607  

Journal: :archives of clinical infectious diseases 0
fernanda milanezi molecular diagnostics laboratory, salomãozoppi diagnostics, sao paulo, brazil ariane falconi molecular diagnostics laboratory, salomãozoppi diagnostics, sao paulo, brazil beatriz schnabel molecular diagnostics laboratory, salomãozoppi diagnostics, sao paulo, brazil luana r. ricardi molecular diagnostics laboratory, salomãozoppi diagnostics, sao paulo, brazil priscilla m. monfredini molecular diagnostics laboratory, salomãozoppi diagnostics, sao paulo, brazil alline t. ziliotto molecular diagnostics laboratory, salomãozoppi diagnostics, sao paulo, brazil

conclusions in conclusion, the data showed that high rate of females harbor these microorganisms in the genital microbiota. this finding suggests the importance of routine gynecological tests, mainly in pregnant females, during the second and third trimesters, and in asymptomatic females under infertility clinical investigation. furthermore, the application of molecular methods is suggested; si...

Journal: :Journal of clinical microbiology 2001
M Schröter B Zöllner P Schäfer R Laufs H H Feucht

The new Light Cycler technology was adapted to the detection of hepatitis C virus (HCV) RNA in clinical samples. Sera from 81 patients were tested by Light Cycler PCR, AMPLICOR HCV Monitor assay, and in-house PCR. Our data demonstrate that Light Cycler is a fast and reliable method for the detection and quantitation of HCV RNA.

Journal: :BioTechniques 2015
William John Andrews Eoin Daniel Brown Margaret Dellett Ruth Esther Hogg David Arthur Simpson

The ability to rapidly detect circulating small RNAs, in particular microRNAs (miRNAs), would further increase their already established potential as biomarkers for a range of conditions. One rate-limiting factor in miRNA detection is the time taken to perform quantitative real-time PCR (qPCR) amplification. We therefore evaluated the ability of a novel thermal cycler to perform this step in le...

Journal: :Clinical chemistry 2015
Andrej-Nikolai Spiess Claudia Deutschmann Michał Burdukiewicz Ralf Himmelreich Katharina Klat Peter Schierack Stefan Rödiger

BACKGROUND Quantification cycle (Cq) and amplification efficiency (AE) are parameters mathematically extracted from raw data to characterize quantitative PCR (qPCR) reactions and quantify the copy number in a sample. Little attention has been paid to the effects of preprocessing and the use of smoothing or filtering approaches to compensate for noisy data. Existing algorithms largely are taken ...

Journal: :Current Biology 1997

Stereomicroscope The new Carl Zeiss stereomicroscope Stemi 2000-CS allows the user to view the sample whilst other observers view the procedure by video. Greenough type stereomicroscopes create their stereo effect by having separate light paths set at different angles, one for each eye. However, when video or photomicrography is being carried out most stereomicroscope systems remove light from ...

Journal: :Current Biology 1997

Stereomicroscope The new Carl Zeiss stereomicroscope Stemi 2000-CS allows the user to view the sample whilst other observers view the procedure by video. Greenough type stereomicroscopes create their stereo effect by having separate light paths set at different angles, one for each eye. However, when video or photomicrography is being carried out most stereomicroscope systems remove light from ...

Journal: :Current Biology 1997

Stereomicroscope The new Carl Zeiss stereomicroscope Stemi 2000-CS allows the user to view the sample whilst other observers view the procedure by video. Greenough type stereomicroscopes create their stereo effect by having separate light paths set at different angles, one for each eye. However, when video or photomicrography is being carried out most stereomicroscope systems remove light from ...

Journal: :Current Biology 1997

Stereomicroscope The new Carl Zeiss stereomicroscope Stemi 2000-CS allows the user to view the sample whilst other observers view the procedure by video. Greenough type stereomicroscopes create their stereo effect by having separate light paths set at different angles, one for each eye. However, when video or photomicrography is being carried out most stereomicroscope systems remove light from ...

Journal: :Current Biology 1997

Stereomicroscope The new Carl Zeiss stereomicroscope Stemi 2000-CS allows the user to view the sample whilst other observers view the procedure by video. Greenough type stereomicroscopes create their stereo effect by having separate light paths set at different angles, one for each eye. However, when video or photomicrography is being carried out most stereomicroscope systems remove light from ...

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