abstract objective : in this study, we have undertaken efforts to ascertain the anticancer potency of s. crispus (sc) extract on diethylnitrosamine (den) and acetylaminofluorene (aaf) induced hcc with special attention to hepatic drug metabolism and to investigate the effect of sc on preneoplastic marker enzyme activity specifically of microsomal aniline hydroxylase (ah) activity and lesion sco...

Hepatic ornithine decarboxylase and aniline hydroxylase activities increased as early as 6h after a single dosing of azathioprine tomale rats and reached maximum levels at the 12th h after the treatment. However, aniline hydroxylase activity dropped to levels mu lower than the cintrols 48 h following azathioprine treatment. The results are discussed with regard to the role of azqathioprine duri...

The influence of a standardized pollen extract (Cernitin) on the hepatic cytochrome P-450 system was studied in rats chronically exposed to an aerosol of ammonium fluoride. The content of cytochrome P-450 and cytochrome b5 and the activities of NADPH-cytochrome c reductase, aminophenazone N-demethylase, and aniline hydroxylase were determined. After 3 months the content of cytochrome b5 in the ...

Effects of lyophilization and storage of rat liver 9,000×g supernatant frac tion or microsomes on activity of aniline hydroxylase, contents of cyt. P-450 and cyt. b5 and aniline-induced P-450 difference spectrum were studied. The lyophiliza tion of rat liver 9,000×g supernatant fraction or microsomes produced about 20% decrease in the activity of aniline hydroxylase. Anil...

Di(2-ethylhexyl) phthalate (DEHP), a commonly used plasticizer and microchemical environmental pollutant, produces subtle changes in hepatic function as judged by increase in liver weight and morphological and biochemical alterations. It can modify the biological response of drugs and other xenobiotics. Such interactions appear to occur at the pharmacokinetic phase, as DEHP was found to alter t...

Hepatic microsomal protein, cytochrome P-450, UDP-glucuronyltransferase, ethylmorphine demethylase, aniline hydroxylase, and aryl hydrocarbon hydroxylase levels were measured in the 2-, 4-, 5-, 6-, and 8-month-old Hanford miniature pig. The activities or concentrations of all of the liver parameters measured had apparently reached their adult plateau level by 2 months of age. The use of the min...

We investigated the effects of trace element deficiency on drug-metabolizing enzymes in rats. Weanling rats were fed a selenium-deficient diet (Torula yeast) or a normal diet (0.5μg selenium) until 20 weeks. After six weeks of the selenium-deficient diet, an increase in aniline hydroxylase activity was observed in the selenium-deficient rats, but the activity of aminopyrine N-demethylase was re...

Components of the hepatic microsomal P-450 hemoprotein electron transfer system (TPNH-cytochrome c reductase and P-450 hemoprotein) and the microsomal cytochrome b5 electron transfer system (DPNH-cytochrome bs reductase and cytochrome bS) were solubilized using Triton N-101 and glycerol, and separated using DEAE-cellulose chromatography. Microsomal aniline hydroxylase activity was reconstituted...

The effects of hydroalcoholic (80% ethanol-20% water) extract of Urtica dioica L. on microsomal aniline 4-hydroxylase (A4H) were investigated in the liver of Swiss albino mice (8- 10-weeks-old) treated with two doses (50 and 100 mg/kg body weight, given orally for 14 days ). The activities of A4H showed a significant increase in the liver at both dose levels of extract treatment. The hydroalcoh...