despite the availability of various alkaline phosphatase (alp) isoenzymes, the calf enzyme is being used in current enzyme assays as the detector enzyme. the glycosylation pattern of this enzyme makes it a suitable ligand for binding to wheat germ agglutinin lectin (wga). as a result of this property, the enzyme can not be used as a conjugate with this lectin, and the calf enzyme conjugates can...

Gold nanoparticles and gold nanoshells functionalised with a selection of carbohydrates: glyco-nanoparticles towards lectin detection in dipstick assay.

Despite the availability of various alkaline phosphatase (ALP) isoenzymes, the calf enzyme is being used in current enzyme assays as the detector enzyme. The glycosylation pattern of this enzyme makes it a suitable ligand for binding to wheat germ agglutinin lectin (WGA). As a result of this property, the enzyme can not be used as a conjugate with this lectin, and the calf enzyme conjugates can...

Modified low-density lipoprotein (LDL) with a low sialic acid content was found in the blood of patients with coronary atherosclerosis. This desialylated lipoprotein causes lipid accumulation in arterial smooth-muscle cells and stimulates cell proliferation and production of the extracellular matrix, i.e., induces all atherogenic manifestations at the cellular level. We have developed a lectin-...

BACKGROUND & OBJECTIVE Mannose binding lectin (MBL), a C-type or Ca(2+) dependent lectin, plays a major role in lectin pathway of complement activation. MBL deficiency/insufficiency is associated with susceptibility to many infections. It is important to know the association of functional lectin levels with disease condition. Therefore, we carried out this study to develop a simple assay to est...

Traditionally, determination of inhibitory potency of complement inhibitors is performed by the hemolytic assay. However, this assay is not applicable to the lectin pathway, thus impeding the understanding of complement inhibitors against the overall function of the complement system. The main objective of our study was to develop a specific enzyme-linked immunosorbent assay (ELISA) as an alter...

Lectins are a glycoprotein that plays significant role in plant and fungi resistance mechanism against insect microorganism attacks. This paper describes for the first time extraction purification of lectin protein found tubers Truffle (Terfezia sp.) by series steps. Initially, precipitated ammonium sulfate accompanied chromatographic techniques include ion-exchange chromatography gel permeatio...