نتایج جستجو برای: leaf explants
تعداد نتایج: 92391 فیلتر نتایج به سال:
An efficient protocol for callus induction and somatic embryogenesis of Cyclamen premium Mill. Was developed. In this study we investigate difference between response of two explants type leaf and petiole and two medium salt concentrations MS and 1⁄2 MS on callus percentage and somatic embryo number. Media was non-significant on callus production but leaf explants was better than petiole and in...
In order to enhance the system of potato transformation and further regeneration, four potato cultivars were transformed using the Agrobacterium tumefaciens harboring â-glucuronidase (GUS) gene.The results revealed that the highest percentages of shoot formation obtained were for Spunta cv. Stem explants resulted in more callus and regeneration after transformation and selection than leaf expla...
To explore the potential for in vitro rapid regeneration of Sainfoin (Onobrychis sativa), different concentrations of 6Benzylaminopurine (BAP), 1-naphthaleneacetic acid (NAA) and BAP combined with Indole-3-butyric acid (IBA) were evaluated for their effects on the induction of somatic embryos from leaf, stem and root explants. Different explants were cultured on MS medium supplemented with vari...
To obtain a higher percentage of callogenesis and consequently the maximum number of plantlets, one of the most important procedures for transgenic plant production. To this end, a factorial experiment using completely randomized design (CRD) with four replications was carried out to assess the effect of cultivar (Marfona and Agria), type of explants(leaf and internode), Kanamycin concentration...
A new protocol was established for the regeneration of Solanum nigrum by frog egg-like bodies (FELBs), which are novel somatic embryogenesis (SE) structures induced from the root, stem, and leaf explants. The root, stem, and leaf explants (93.33%, 85.10%, and 100.00%, respectively) were induced to form special embryonic calli on Murashige and Skoog (MS) medium containing 1.0 mg/L 2,4-dichloroph...
بابونه شفاف یا anthemis hyalinedcیکی از مهم ترین گیاهان دارویی بوده که اسانس آن در صنایع مختلف داروسازی وآرایشی-بهداشتی مورد استفاده قرار می گیرد. مطالعاتی در زمینه کشت بافت این گونه از بابونه صورت نگرفته است و با توجه به خصوصیات دارویی و ترکیبات اسانس بابونه شفاف، مطالعات کشت درون شیشه این گیاه می تواند دارای اهمیت باشد. در این مطالعه تأثیر سطوح مختلف هورمون های گیاهی نفتالین استیک اسید(0 ، 1/...
Induction of somatic embryogenesis in absence of exogenous auxin can clarify some basic concepts of somatic embryogenesis. Explants from leaf and petiole of Cucumber (Cucumis sativus) were used in this experiment for somatic embryogenesis using three different modified B5 basal media; boron and 2,4-D but no IAA as control (I), boron and IAA but no 2,4-D (II) and no boron, 2,4-D and IAA (III). ...
Astragalus species are medicinal plants which produce valuable secondary metabolites, especially cycloartane-type glycosides. In this study, stem and leaf explants of Astragalus trojanus were subjected to different plant growth regulators, environmental conditions and media compositions to identify their callus responses. Stem and leaf explants were cultured in Murashige and Skoog (MS) and wood...
Abstract—Stevia rebaudiana Bertoni (natural sweetener) belongs to Asteraceae family and can be used as substitute of artificial sweeteners for diabetic patients. Conventionally, it is cultivated by seeds or stem cutting, but seed viability rate is poor. A protocol for callus induction and multiplication was developed to produce large no. of calli in short period. Surface sterilized nodal, leaf ...
In micropropagation of Anthurium the most important and sensitive stage, is callus induction from explant. This experiment was conducted in a completely randomized design with factorial arrangement in 4 replications in Sari Agriculture Sciences and Natural Resources University (SANRU) in 2010. The cultivar used in this study is Fire and leaf segments with and without main nervure, were used as ...
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