In plants, multiple detached tissues are capable of forming a pluripotent cell mass, termed callus, when cultured on media containing appropriate plant hormones. Recent studies demonstrated that callus resembles the root-tip meristem, even if it is derived from aerial organs. This finding improves our understanding of the regeneration process of plant cells; however, the molecular mechanism tha...

A methanol extract of Gymnema sylvestre leaf and callus showed anti-diabetic activities through regenerating β-cells. Optimum callus was developed under stress conditions of blue light with 2,4-D (1.5 mg/l) and KN (0.5 mg/l), which induced maximum biomass of green compact callus at 45 days, as determined by growth curve analysis. Leaf and optimum callus extracts contains gymnemic acid, which wa...

As a measure of testing the medicinal properties of Jatropha curcas, methanol extract obtained from both in vivo leaf and leaf derived callus were subjected to antimicrobial activity against six microorganisms, of the six different concentrations tested, the in vitro leaf callus extracts of at high concentrations (1.0 and 1.2%) inhibited the growth of Staphylococcus aureus and Pseudomonas sp. a...

In the present study, antioxidant activities of the three plant parts (stem, leaf and callus) were investigated. Ethanolic extracts of stem, leaf and callus powder was characterized by the method described by (Hatano et al., 1988) in which stable 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical solution was used. In this paper, it is found that all of the in vitro and in vivo extracts have re...

The leaf of Gardenia jasminoides Ellis was used as explants and was cultured on MS and WPM media supplemented with various concentrations of NAA, IAA, 2,4-D, IBA, TDZ, and Kn (0 to 5 mg L(-1) with 0.5 increment). After six months, the higher percentage of callus (100%) and the best dry and fresh weight of callus were formed on WPM medium supplemented with 2,4-D and NAA (2.0-3.0 mg L(-1)) and th...

An efficient protocol for callus induction and somatic embryogenesis of Cyclamen premium Mill. Was developed. In this study we investigate difference between response of two explants type leaf and petiole and two medium salt concentrations MS and 1⁄2 MS on callus percentage and somatic embryo number. Media was non-significant on callus production but leaf explants was better than petiole and in...

This experiment was performed to optimize the medium for tissue culture of Taraxacum kok-saghyz Rodin. Different tissue culture approaches such as shoot regeneration from seed, callus formation from leaf explants and plant regeneration from callus were investigated in this study. All the explants were cultured on MS basal medium supplemented with 20g/l sucrose, 7g/l agar and different plant gro...

Radopholus similis and Pratylenchus coffeae were reared on callus and roots developed from citrus leaves. Callus formed best when leaf petioles were immersed in Astatula fine sand and the leaves were sprayed daily with 4 ppm 2,4-D solution and maintained at 25 or 30 C. The nematodes completed one generation in 20 days at 25 C. Highest populations of R. similis (1,127) occurred after 50 days, an...

Unorganized callus and leaf/root-differentiating callus cultures of Pimpinella major have been established in liquid nutrient medium. Their capacity to accumulate rare phenylpropa­ noids such as epoxy-pseudoisoeugenol tiglate, epoxy-anol tiglate and anol tiglate was com­ pared with that of seedlings and whole plants. The unorganized callus cultures were not able to accumulate any phenylpropanoi...

Different sugarcane varieties (CP 77/400, CP 85/1491 and Punjabi) were checked for its callus induction and regeneration capabilities using different growth regulators and explants (eye bud, upper portion of inner leaf whorls and lower portion of inner leaf whorls). Seven callus induction media i.e. CIM-1 to CIM-6 and control media, five shoot induction media i.e. SIM-1 to SIM-4 and control med...