introduction: implantation of transferred embryos in the uterus after ivf_icsi cyclesis an important process that less information about it is available. zona hatching method is a suggested approach for this goal. the aim of this research is about performance of zona hatching by laser. methods: in this study 32 patients that enrolled had ivf/icsi cycles depend on inclusion criteria of study tha...

objective: the aim of this study was to investigate the effect of laser assisted hatching on the development and quality of vitrified-warmed 4-cell stage mouse embryos. materials and methods: the vitrified-warmed 4-cell mouse embryos were divided into two groups: control group (without laser assisted hatching) and experiment group (with laser assisted hatching). all embryos in both groups were ...

background: routine use of assisted hatching (ah) following icsi is a controversial issue in the literature. there are rare studies regarding the effect of laser assisted hatching (lah) on live birth rate. objective: our main goal was to evaluate the effect of lah on delivery rate as well as congenital anomaly in patients undergoing their first icsi cycle. materials and methods: a total of 182 ...

OBJECTIVE In search of an ideal method of assisted hatching (AH), we compared the effects of conventional micropipette-AH and laser-AH on the blastocyst formation rate (BFR) and blastocyst cell numbers. METHODS Four- to five-week-old ICR female mice were paired with male mice after superovulation using Pregnant mare's serum gonadotropin (PMSG) and hCG. The two-cell embryos were flushed from t...

abstract background: for infertile women aged over 35 years, failure of the zp (zona pellucida) to rupture is believed to be associated with a decreased implantation rate in in vitro fertilization (ivf) or intra cytoplasmic sperm injection (icsi). objective: in this research, laser assisted hatching (lah) was offered to patients with advanced maternal age to evaluate a possible benefit. materia...

The Use of Laser-Assisted Hatching in Bovine in Vitro Produced Embryos to Improve Pregnancy Rate. (August 2008) Suzanne Lynn Menges, B.S., Texas A&M University Chair of Advisory Committee: Dr. Duane Kraemer In vitro produced (IVP) embryos not hatching from the zona pellucida (ZP) after transfer is one possible contributing factor of a lower pregnancy rate when compared to in vivo embryos. This ...

BACKGROUND In MII oocytes showing difficult oolemma breakage, ICSI can cause an increase in the degeneration rate. This may be overcome by laser-assisted ICSI using a 5-10 micro m opening in the zona pellucida for injection. However, such a small opening might impair the hatching process, especially if assisted hatching is applied in addition. In order to prevent this, the present study used ro...