objective: the aim of this study was to investigate the effect of laser assisted hatching on the development and quality of vitrified-warmed 4-cell stage mouse embryos. materials and methods: the vitrified-warmed 4-cell mouse embryos were divided into two groups: control group (without laser assisted hatching) and experiment group (with laser assisted hatching). all embryos in both groups were ...

abstract background: for infertile women aged over 35 years, failure of the zp (zona pellucida) to rupture is believed to be associated with a decreased implantation rate in in vitro fertilization (ivf) or intra cytoplasmic sperm injection (icsi). objective: in this research, laser assisted hatching (lah) was offered to patients with advanced maternal age to evaluate a possible benefit. materia...

background: routine use of assisted hatching (ah) following icsi is a controversial issue in the literature. there are rare studies regarding the effect of laser assisted hatching (lah) on live birth rate. objective: our main goal was to evaluate the effect of lah on delivery rate as well as congenital anomaly in patients undergoing their first icsi cycle. materials and methods: a total of 182 ...

OBJECTIVE In search of an ideal method of assisted hatching (AH), we compared the effects of conventional micropipette-AH and laser-AH on the blastocyst formation rate (BFR) and blastocyst cell numbers. METHODS Four- to five-week-old ICR female mice were paired with male mice after superovulation using Pregnant mare's serum gonadotropin (PMSG) and hCG. The two-cell embryos were flushed from t...

BACKGROUND In MII oocytes showing difficult oolemma breakage, ICSI can cause an increase in the degeneration rate. This may be overcome by laser-assisted ICSI using a 5-10 micro m opening in the zona pellucida for injection. However, such a small opening might impair the hatching process, especially if assisted hatching is applied in addition. In order to prevent this, the present study used ro...

Problem statement: The ability of a blast cyst to hatch, or escape, from the Zona Pellucida (ZP) that surrounds and protects the embryo during its first few days of development is one of many critical events that must occur for successful reproduction. Implantation of the embryo in the uterine lining is impossible unless hatching occurs. Hard or thick ZP will therefore impaire the implantation ...

BACKGROUND Extended in vitro embryo culture and blastocyst transfer have emerged as essential components of the advanced reproductive technology armamentarium, permitting selection of more advanced embryos considered best suited for transfer. AIM OF STUDY The aim of this study was to compare between cleavage stage and blastocyst stage embryo transfer in patients undergoing intracytoplasmic sp...

STUDY QUESTION Does laser-assisted zona thinning of cleavage stage mouse embryos facilitate hatching in vitro? SUMMARY ANSWER No, unlike laser zona opening, zona thinning does not facilitate embryo hatching. WHAT IS KNOWN ALREADY Artificial opening of the zona pellucida facilitates hatching of mouse and human embryos. Laser-assisted zona thinning has also been used for the purpose of assist...

The Use of Laser-Assisted Hatching in Bovine in Vitro Produced Embryos to Improve Pregnancy Rate. (August 2008) Suzanne Lynn Menges, B.S., Texas A&M University Chair of Advisory Committee: Dr. Duane Kraemer In vitro produced (IVP) embryos not hatching from the zona pellucida (ZP) after transfer is one possible contributing factor of a lower pregnancy rate when compared to in vivo embryos. This ...