نتایج جستجو برای: labeled fluorescence in situ hybridization
تعداد نتایج: 17005930 فیلتر نتایج به سال:
background: it has been claimed that by using different washing methods, the sperms can be separated according to size, motility, density, chromosomal content and surface markings and charge. these methods also reduce sperm chromatin deficiencies and screen the sperms before applying in assisted reproduction techniques. objective: this study compared simple density gradient methods and a combin...
In situ hybridization (ISH) is a method that uses labeled complementary single strand DNA or RNA to localize specific DNA or RNA sequences in an intact cell or in a fixed tissue section. The main steps of ISH consist of: probe selection, tissue or sample preparation, pre-hybridization treatment, hybridization and washing, detection and control procedure. Probe selection is one of the important ...
پس از شرح مختصری در مورد تولد دانش سیتوژنتیک (1956) و کاربرد آن در پزشکی (1959) به عنوان آزمایش ارزنده ای برای تشخیص بیماریهای ارثی ناشی از ناهنجاریهای کروموزومی، محدودیت دامنه کارآیی آن، محققین را بر آن داشت روش های نواری banding را کشف و به کار گیرند. این روش توانست بسیاری از مشکلات و محدودیت های قبلی را مرتفع نماید ولی باز در مواردی به ویژه در نمونه هایی که امکان کشت بافت مقدور نبود، همچنین...
in this thesis a calibration transfer method is used to achieve bilinearity for augmented first order kinetic data. first, the proposed method is investigated using simulated data and next the concept is applied to experimental data. the experimental data consists of spectroscopic monitoring of the first order degradation reaction of carbaryl. this component is used for control of pests in frui...
perrault's syndrome (p.s.) is rare. the combination of gonadal dysgenesis and hearing loss was accompanied by 46,xxkaryotype in three sisters with parental consanguineous marriage. genetic investigation revealed normal female karyotype, positive liarr and negative fluorescence ipj-bodies, which was confirmed by molecular study on the basis of fluorescence in situ hybridization (pish), with appl...
in this study, the bacterial diversity in the activated sludge system of a full-scale municipalwastewater treatment plant in dubai was monitored over a period of one year using ribosomal rna (rrna)targeted oligonucleotide probes for a defined phylogenetic group of bacteria by the fluorescence in situhybridization (fish) technique. the largest fraction of the bacterial community in the sludge sa...
in this study, the bacterial diversity in the activated sludge system of a full-scale municipalwastewater treatment plant in dubai was monitored over a period of one year using ribosomal rna (rrna)targeted oligonucleotide probes for a defined phylogenetic group of bacteria by the fluorescence in situhybridization (fish) technique. the largest fraction of the bacterial community in the sludge sa...
Estimation of absorbed dose for radiation workers or person involved in different radiological accident is the aim of biodosimetry. Cytogenetic methods are the most current and applicable biodosimetry tools. In chronic or protracted exposure, fluorescence in situ hybridization (FISH), stable chromosomal aberration is used for estimation of absorbed dose. For precise estimation of absorbed dose,...
This study was conducted to determine the location of DNA segment with homology to the rat conserved genomic DNA in human chromosomes. The labeled rat genomic DNA was hybridized with normal human (male) metaphases. The study of 74 metaphases after fluorescence in situ hybridization showed 371 twin-spot signals on human chromosomes. Statistical analysis indicated that the specific accumulation o...
background: various fixation and permeabilization techniques have been developed for detection of intracellular antigens by flow cytometry; however, there are few studies using flow cytometry to detect the frequency of intracellular nucleic acids, particularly rna. we tested six different permeabilization methods in order to gain access to a high quality method with minimal damage to intracellu...
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