نتایج جستجو برای: ku70

تعداد نتایج: 763  

Journal: :Clinical cancer research : an official journal of the American Association for Cancer Research 2013
Firas L T Al-Ubaidi Niklas Schultz Olga Loseva Lars Egevad Torvald Granfors Thomas Helleday

PURPOSE Neoadjuvant castration improves response to radiotherapy of prostate cancer. Here, we determine whether castration therapy impairs nonhomologous end-joining (NHEJ) repair of DNA double-strand breaks (DSB) by downregulating Ku70 protein expression. EXPERIMENTAL DESIGN Twenty patients with locally advanced prostate cancer were enrolled, and 6 to 12 needle core biopsy specimens were take...

2014
Yong Jun Choi Han Li Mi Young Son Xiao-hong Wang Jamie L. Fornsaglio Robert W. Sobol Moonsook Lee Jan Vijg Sandra Imholz Martijn E. T. Dollé Harry van Steeg Erwin Reiling Paul Hasty

Ku70 and Ku80 form a heterodimer called Ku that forms a holoenzyme with DNA dependent-protein kinase catalytic subunit (DNA-PKCS) to repair DNA double strand breaks (DSBs) through the nonhomologous end joining (NHEJ) pathway. As expected mutating these genes in mice caused a similar DSB repair-defective phenotype. However, ku70(-/-) cells and ku80(-/-) cells also appeared to have a defect in ba...

Journal: :Molecular cancer research : MCR 2013
Chitra Subramanian Manila Hada Anthony W Opipari Valerie P Castle Roland P S Kwok

Ku70 was originally described as an autoantigen, but it also functions as a DNA repair protein in the nucleus and as an antiapoptotic protein by binding to Bax in the cytoplasm, blocking Bax-mediated cell death. In neuroblastoma (NB) cells, Ku70's binding with Bax is regulated by Ku70 acetylation such that increasing Ku70 acetylation results in Bax release, triggering cell death. Although regul...

Journal: :Experimental cell research 2006
Vivian Gama Tomoyuki Yoshida Jose A Gomez David P Basile Lindsey D Mayo Arthur L Haas Shigemi Matsuyama

Ku70 plays an important role in DNA damage repair and prevention of cell death. Previously, we reported that apoptosis caused a decrease in cellular Ku70 levels. In this study, we analyzed the mechanism of how Ku70 levels decrease during drug-induced apoptosis. In HeLa cells, staurosporin (STS) caused a decrease in Ku70 levels without significantly affecting Ku70 mRNA levels. We found that Ku70...

2014
Manila Hada Roland PS Kwok

Ku70, a DNA repair factor in the nucleus, also regulates cell death by binding to the apoptotic protein Bax in the cytoplasm. Acetylation of Ku70 triggers Bax release resulting in Bax dependent cell death. Thus dissociating Bax from Ku70, either by inhibiting histone deacetylase 6 (HDAC6) that deacetylates Ku70 or by increasing Ku70 acetylation induces cell death. Our results showed that in neu...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1997
Y Gu S Jin Y Gao D T Weaver F W Alt

V(D)J recombination requires both lymphoid-specific and generally expressed enzymatic activities. All three known generally expressed activities involved in V(D)J recombination are also involved in DNA double-strand break repair (DSBR). Two of these are components of the DNA-dependent protein kinase (DNA-PK) and include Ku80 and DNA-PK catalytic subunit (DNA-PKcs); the third, XRCC4, is a protei...

Journal: :Cell 2005
Juan J. Martinez Stéphanie Seveau Esteban Veiga Shigemi Matsuyama Pascale Cossart

Rickettsia conorii, a strictly intracellular and category C priority bacterial pathogen (NIAID), invades different mammalian cells. Although some signaling events involved in bacterial entry have been documented, the bacterial and host proteins mediating entry were not known. We report the identification of the Ku70 subunit of DNA-dependent protein kinase (DNA-PK) as a receptor involved in R. c...

Journal: :Molecular Cell 1998

Journal: :The Journal of veterinary medical science 2013
Manabu Koike Yasutomo Yutoku Aki Koike

The DNA repair protein Ku70 is a key player in chemoresistance to anticancer agents (e.g., etoposide) or radioresistance. The responses of different organs to radiation vary widely and likely depend on the cell population in the organs. Previously, we established and characterized Ku70-deficient murine lung epithelial (Ku70 -/- MLE) cells and found that these cells are more sensitive than Ku70 ...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2008
Avigail D Amsel Moran Rathaus Noam Kronman Haim Y Cohen

The DNA end-joining protein Ku70 is one of several proteins that inhibit apoptosis by sequestering the proapoptotic factor Bax from the mitochondria. However, the molecular mechanism underlying Ku70-dependent inhibition of Bax is not fully understood. Here, we show that the absence of Ku70 results in the accumulation of ubiquitylated Bax. Under normal growth conditions, Bax ubiquitylation promo...

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