The methodology and principle using vibrational energy transfer to measure molecular distances in liquids are introduced. The application of the method to the studies of ion pairing and clustering in strong electrolyte aqueous solutions is demonstrated with MSCN aqueous solutions where M = Li, Na, K, Cs, and NH4. Experiments suggest that ions in the concentrated aqueous solutions can form subst...

The title compound (1) was crystallized as free ligand and as its (1 • KSCN)2 • H 2 0 complex. Crystallographic data are, for the former: space group C 2/c, a — 31.110(9), b = 4.608(4), c = 17.188(6) A, ß = 105.27(8)°, Z = 2, Dm = 1.25 gem-3 ; for the latter: space group P2i, a = 11.044(3), b = 18.310(4), c = 14.485(4) Ä, ß = 97.49(2)°, Z = 4, Dm = 1.27 gem -3 . Crystal structures were solved b...

Certain Escherichia coli tryptophan synthase mutant alpha-subunits encoded from mutagenized trpA-containing plasmids were overexpressed as insoluble aggregates which were seen as large, intracellular inclusion bodies. The insoluble aggregates were solubilized to various degrees by several neutral, chaotropic salts. The order of effectiveness of these salts (KSCN, NaI greater than NaNO3, LiBr gr...

In general, intermolecular distances in condensed phases at the angstrom scale are difficult to measure. We were able to do so by using the vibrational energy transfer method, an ultrafast vibrational analogue of Förster resonance energy transfer. The distances among SCN(-) anions in KSCN crystals and ion clusters of KSCN aqueous solutions were determined with the method. In the crystalline sam...

The thiocyanate (SCN(-)) anion is known as one of the best denaturants, which is also capable of breaking the hydrogen-bond network of water and destabilizing native structures of proteins. Despite prolonged efforts to understand the underlying mechanism of such Hofmeister effects, detailed dynamics of the ions in a highly concentrated solution have not been fully elucidated yet. Here, we used ...

Influences of different concentrations of salts (0.002 to 1.25 eq per liter) on the binding of skatole and acetyl-Ltryptophan at the primary binding site of bovine plasma albumin have been studied by equilibrium dialysis. With the exception of Naz-ethylenediaminetetraacetate and ethylenediammonium aspartate, which have little effect, all salts in the concentration range of 0.005 to 0.1 eq per l...

The electrochemical and corrosion behaviour of Al-6061, Al-4.5%Cu and Al-7.5%Cu alloys were studied in 0.50 M KSCN solution at 25 C. Open-circuit potential, Tafel polarization and ICP-AES measurements were used to study the uniform corrosion process on the surfaces of the tested alloys. Cyclic polarization measurements were employed for pitting corrosion studies. Obtained results were compared ...

KSCN and NH4SCN aqueous solutions were investigated with intermolecular vibrational energy transfer methods. In a KSCN/H2O (1/10 molar ratio) solution, 90% of the initial excitation of the CN stretch (~2066 cm(-1)) of the SCN(-) anion is transferred to the HOH bending mode (~1636 cm(-1)) of water molecules with an energy transfer time constant 3.1 ps. In a NH4SCN/H2O (1/10 molar ratio) solution...