To perform PCR from serum for the diagnosis of visceral leishmaniasis is convenient and much less invasive than examination deeper compartments such as bone marrow. We compared three Leishmania-specific real-time PCRs with different molecular targets (kinetoplast DNA, small subunit-ribosomal RNA-(ssrRNA-)gene, glucose-6-phosphate isomerase-(gpi-)gene) regarding their sensitivity specificity in ...

The loss of kinetoplast DNA in Leishmania tarentolae, which occurs in the presence of low concentrations of acriflavin, was found to be a result of selective inhibition of replication of this DNA. Nuclear DNA synthesis was relatively unaffected and cell and kinetoplast division proceeded normally for several generations. An approximately equal distribution of parental kinetoplast DNA between da...

The loss of kinetoplast DNA in Leishmania tarentolae, which occurs in the presence of low concentrations of acriflavin, was found to be a result of selective inhibition of replication of this DNA. Nuclear DNA synthesis was relatively unaffected and cell and kinetoplast division proceeded normally for several generations. An approximately equal distribution of parental kinetoplast DNA between da...

The loss of kinetoplast DNA in Leishmania tarentolae, which occurs in the presence of low concentrations of acriflavin, was found to be a result of selective inhibition of replication of this DNA. Nuclear DNA synthesis was relatively unaffected and cell and kinetoplast division proceeded normally for several generations. An approximately equal distribution of parental kinetoplast DNA between da...

The mitochondrial DNA of Trypanosoma brucei is organized in a complex structure called the kinetoplast. In this study, we define the complete kinetoplast duplication cycle in T. brucei based on three-dimensional reconstructions from serial-section electron micrographs. This structural model was enhanced by analyses of the replication process of DNA maxi- and minicircles. Novel insights were obt...

In Critliidia fascicuJata division of the kinetoplast occurs prior to karyokinesis. Kinetoplast division involves the lateral replication of the nucleoid, and the subsequent constriction and 'pinching off' of the nucleoid and mitochondrial components. Two subunits are produced. DNA synthesis in the kinetoplast and the nucleus is overlapping but asynchronous. Experiments for short labelling time...

Trypanosomes have an unusual mitochondrial genome, called kinetoplast DNA, that is a giant network containing thousands of interlocked minicircles. During kinetoplast DNA synthesis, minicircles are released from the network for replication as theta-structures, and then the free minicircle progeny reattach to the network. We report that a mitochondrial protein, which we term p38, functions in ki...

objective: ajowan is an annual herbaceous essential oil of carum copticom. the main components of the oil are tymol, β-pinene, γ- terpinene and sabinene.the fruit oil of carum copticum has been reported to have several therapeutic effects including anti fungal, anti bacterial and anti viral,... candida albicans is an opportunistic fungus and transforms into pathogenic form in favorable conditio...

The electron microscopical aspect of kinetoplast DNA has been studied in preparations obtained by osmotic disruption of isolated organelles. A large amount of this DNA appears to be of large molecular size. This is apparently at variance with the observations, made by others using a different technical approach, of kinetoplast DNA consisting mainly of small circular molecules of constant size. ...

Members of the protozoan order Kinetoplastida (Honigberg et al., 1964), which includes the genera Trypanosoma, Leishmania, and Crithidia, are characterized by the presence of a body known as a kinetoplast. The kinetoplast is a modified region of a mitochondrion (Meyer et al., 1958; Steinert, 1960; Clark and Wallace, 1960) which contains so much DNA that it can be detected by Feulgen staining (B...