Two types of molecular markers, random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR), were assayed to determine the genetic diversity of 46 barley accessions, including 27 landraces of H. vulgare ssp vulgare (HV), 6 accessions of H. vulgare ssp. spontaneum (HS) and 13 accessions of H. vulgare ssp. agriocrithon (HA), from west China. A high level of polymorphism was fo...

Molecular characterization of eleven clones of C. winterianus collected from eleven districts of West Bengal was carried out with 10 RAPD and 9 ISSR primers. The RAPD primers developed 81 robust loci, which revealed 74.07% polymorphism and 61 ISSR markers generated 47.50% polymorphism. Out of the different anchored primer combinations, ISSR primers with AG and GA motifs produced clear and maxim...

Two types of molecular markers, random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR), were assayed to determine the genetic diversity of ten experimental materials in Rehmannia glutinosa Libosch .f. hueichingensis (Chao et Schih) Hsiao, including eight cultivars and two virus-free lines micropropagated by tip tissue culture from Wenxian county. A high level of polymor...

Fusarium Head Blight is an important wheat disease in the Argentine Pampas region, being Fusarium graminearum the predominant pathogen. DNA polymorphism of the isolates was analyzed by IGS-RFLP and ISSR. IGS-RFLP and ISSR profiling were carried out using six endonucleases and eight primers, respectively. IGS-RFLP yielded 41 bands, 30 of which were polymorphic while ISSR produced 87 bands with 4...

Kumar A, Verma N. 2012. A comparative phylogenetic analysis of medicinal plant Tribulus terrestris in Northwest India revealed by RAPD and ISSR markers. Biodiversitas 13: 107-113. Several DNA marker systems and associated techniques are available today for fingerprinting of plant varieties. A total of 5 RAPD and 8 ISSR primers were used. Amplification of genomic DNA of the 6 genotypes, using RA...

The genetic diversity in Zanthoxylum species viz.  Zanthoxylum nitidum, Zanthoxylum oxyphyllum and Zanthoxylum rhesta collected from the Upper Brahmaputra Valley Zone of Assam (NE India) was amplified using 13 random amplified polymorphic DNA (RAPD) markers and 9 inter-simple sequence repeat (ISSR) markers. RAPD markers were able to detect 81.82% polymorphism whereas ISSR detected 98.02% polymo...

In the present study, 20 sandalwood (Santalum album L.) genotypes were characterized using RAPD, ISSR and SSR markers. Twenty-five RAPD and twenty-one ISSR primers that generated clear and reproducible banding patterns amplified 225 and 208 bands, respectively, among 20 sandalwood genotypes. Out of 225, 181 (83.13 %) RAPD bands were polymorphic while out of 208, 156 (75.77 %) ISSR bands were po...

Sequence-related amplified polymorphism (SRAP) and inter-simple sequence repeat (ISSR) markers were used to assess the genetic diversity within and among 15 natural populations of Stipa bungeana from the Loess Plateau of China. Using 15 SRAP primers, 504 (99.80%) polymorphic loci were detected, and 372 polymorphic loci (96.12%) were identified using 15 ISSR primers. At the species level, the S....

Cotton is one of the most economically important crops in Iran; hybridization is a means to increase the genetic diversity and obtain new elite cultivars in this crop. We examined agronomic characteristics and molecular genetic diversity in the Opal cotton (Gossypium hirsutum) cultivar and in F(2) progenies. Ten homo-primers and seven hetero-primers of 26 RAPD primers produced 261 reproduc...

Morphologically, nine different slow-growing protoclones were screened from regenerated protoplast of heterokaryotic Agaricus bisporus. The present study is the first report of fingerprinting on differentiating homo- and heterokaryotic protoclones using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. Among 80 primers tested, the seven ISSR and seven RAPD...