Twenty-five Landrace • Yorkshire sows (second through seventh parity) were randomly assigned to five dietary treatments in an experiment designed to estimate the isoleucine requirement of the sow during the first 3 weeks of lactation. The basal diet (.20% isoleucine), composed of oat groats, corn sugar, wood cellulose, soybean oil, vitamins, minerals, indispensable amino acids and L-glutamic ac...

amino acid dehydrogenases (l-amino acid: oxidoreductase deaminating ec 1.4.1.x) are members of the wider superfamily of oxidoreductases that catalyze the reversible oxidative deamination of an amino acid to its keto acid and ammonia with the concomitant reduction of either nad+, nadp+ or fad. these enzymes have been received much attention as biocatalysts for use in biosensors or diagnostic kit...

Amino acid dehydrogenases (L-amino acid: oxidoreductase deaminating EC 1.4.1.X) are members of the wider superfamily of oxidoreductases that catalyze the reversible oxidative deamination of an amino acid to its keto acid and ammonia with the concomitant reduction of either NAD+, NADP+ or FAD. These enzymes have been received much attention as biocatalysts for use in biosensors or diagnostic kit...

A 9-week feeding trial was conducted in low temperature season to evaluate the effects of varying levels of dietary isoleucine on hematology and innate immune response of juvenile olive flounder. Six isonitrogenous (45% crude protein) and isocaloric (4.45 kcal g gross energy) diets containing graded levels of isoleucine (0.48, 0.87, 1.43, 1.94, 2.37 and 2.78% dry diet) were formulated. Triplica...

Whole cells of Corynebacterium glutamicum were loaded with high cytoplasmic l-isoleucine concentrations, and isoleucine excretion from these cells was studied in terms of mechanism and regulation. The transmembrane isoleucine flux could be differentiated into carrier-mediated uptake, carrier-mediated excretion, and diffusion. After discrimination from the other transmembrane solute movements, t...

The emission of light in the marine ostracod Cypridina hilgendorfii (presently Vargula hilgendorfii) is produced by the Cypridina luciferin-luciferase reaction in the presence of molecular oxygen. Cypridina luciferin has an asymmetric carbon derived from isoleucine, and the absolute configuration is identical to the C-3 position in L-isoleucine or D-alloisoleucine. To determine the stereoselect...

When dl-isoleucine was added to an ammonium nitrate-maltose medium during cultivation of Streptomyces sp. 732, quinomycin B formation was selectively enhanced (from 3 to 70% of the quinomycin mixture) and quinomycin C synthesis was inhibited completely. In addition, two new quinomycins, designated quinomycins D and E, were produced in the presence of isoleucine. These compounds were found to co...