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background: in vitro model studies are becoming increasingly popular for experimental research designs. they include isolation and expansion of cells of a particular tissue, such as the nervous tissue which contributes to understanding the underlying mechanisms in many pathologies. it enables  the scrutinization of intracellular signaling pathways responsible for cell death. objectives: in the ...

abstract background and aims: to identify and isolation of viruses a susceptible cell is need which can be primary or line. the use of primary cells is laborious, time consuming and expensive, while the use of cell lines is much easier and faster. irkhbk cell line was originated from primary cells of bovine kidney by continuous passage. the aim of this study was to introduce the irkhbk cell lin...

background: in cancer patients, chemo and radiotherapy can cause infertility by damaging spermatogenesis process. this process is based on self-renewal and differentiation of a rare population of the testicular cells called spermatogonial stem cells (sscs). scientists have tried to isolate, enrich and culture human spermatogonial stem cells, hoping to resolve infertility problems in cancer reco...

Introduction:Liver has important roles in body metabolic regulation and for this reason hepatocytes are used worldwide.Investigations showed that isolation of hepatocytes causes activation of stress related genes.The aim of this study was to study the stress related expression of BEST-5 following hepatocytes isolation and culture. Method:The BEST-5 gene is cloned and analyzed for the first tim...

Cell isolation and culture are essential tools for the study of cell function. Isolated cells grown under controlled conditions can be manipulated and imaged at a level of resolution that is not possible in whole animals or even tissue explants. Recent advances have allowed for large-scale isolation and culture of primary C. elegans cells from both embryos and all four larval stages. Isolated c...

The aetiology of inflammatory bowel disease is at present obscure, but there is evidence that transmissible agents such as bacteria or viruses may be associated with this condition.' 4 Chlamydia trachomatis agents are known to infect the rectum5 6 and lymphogranuloma venereum (LGV) serotypes can cause chronic inflammation with fistula formation similar to manifestations of Crohn's disease. Prev...