background: leishmaniasis is endemic in iran. different species of leishmania (l.) parasites are causative agents of this disease. correct identification of leishmania species is important for clinical studies, prevention, and control of the diseases. mix up of leishmania isolates is possible in the laboratory, so there is need for verification of species for isolates of uncertain identity. dif...

during the period from 1996 to 1997, 45 isolates of leishmania were recovered frompatients suspected of cutaneous leishmaniasis (cl) and one from a gerbil. these specimens werereceived from various parts of iran. isoenzyme profiles of these isolates were compared with those ofreference strains of l.tropica, l.major and l.infantum using cellulose acetate electrophoresis (cae) and10 enzyme system...

both zoonotic and anthroponotic cutaneous leishmaniasis (cl) caused by l. major and l. tropica, respectively, are endemic in different parts of iran. this study was performed to investigate the new changes in epidemiological pattern of cl, and to identify the species of leishmania and the strains of l. major isolated from different endemic areas of iran. seventy-two isolates from 245 samples co...

The method is based on the selective activating capacity of dithiothreitol on creatine kinase isoenzyme MB, after isoenzyme MM is activated by glutathione. Isolated isoenzymes MM and MB of human and canine origin were assayed individually and in mixtures of known activities. When glutathione was present in the assay medium the activity of each isoenzyme could be measured individually, but gluta...

The aim of the study was to evaluate usefulness determining markers myocardial damage, such as: aspartate aminotransferase, total creatine kinase, cardiac kinase isoenzyme, lactate dehydrogenase, dehydrogenase isoenzyme and peripheral blood lactic acid in sport horses subjected physical exertion. research carried out on 50 three breeds: Ukrainian Riding, Hanoverian Westphalian, aged 4-16 years....

In this study, we used Random Amplified Polymorphic DNA (RAPD) for identification of 17 isolates of Leishmania from the skin and reticuloendothelial system of humans, animal reservoirs (rodent and dog) and sandflies in various parts of Iran in the last decade. Fifteen species have been confirmed by isoenzyme characterization by the London School of Hygiene and Tropical Medicine and Shiraz ...

Serum lacticodehydrogenase (LDH) is commonly increased in patients with haematopoietic malignancies and has been shown to be a prognostic factor in patients with non-Hodgkin's lymphoma (NHL) and myeloma. We have examined the LDH isoenzyme content in serum of 326 patients, including 252 patients with NHL (202 at diagnosis and 50 at relapse), 28 patients with Hodgkin's disease, 17 patients with C...

Human isoenzyme LDH-X (lactate dehydrogenase isoenzyme X) was isolated from seminal fluid of frozen semen samples by affinity chromatography by using oxamate-Sepharose and AMP-Sepharose. In the presence of 1.6 mM-NAD+, isoenzyme LDH-X does not bind to AMP-Sepharose, whereas the other lactate dehydrogenase isoenzymes do. This is the crucial point in the isolation of isoenzyme LDH-X from the othe...

Fig. 1. Electropherogram stained for alkaline phosphatase isoenzymes A. albumin with added biiWubin; B, billrubin solution In saline; C, patient’s serum with artifactual band in addition to placental and lIver isoenzyme bands (poody resolved); D. intestinal and placental isoenzyme control; E, patient’s serum with liver Isoenzyme band; F, Intestinal and live’ lsoenzyme control; 0, patient’s seru...

Serum and tumor tissue of a patient with neuroblastoma contained an abnormal isoenzyme of lactate dehydrogenase (LDH; EC 1.1.1.27), which, on agarose gel electrophoresis, migrated between LDH-2 and LDH-3 with a mobility the same as that of the extra LDH isoenzyme found in normal human erythrocytes. On surgical removal of the tumor, the high total LDH activity (775 U/L) in the serum of the patie...