liver is one of the most important organs needing great concern during the perioperative period. there are a number of different mechanisms that interact with liver cells and might affect their integrity and cell live. though these mechanisms are not all the same, there is a great common point: all affect the metabolic pathways of the liver. ischemia, anesthetic drug effects and other periopera...

Solubilization of the human erythrocyte membrane by seven detergents is described. Components released into the supernatant or retained in the residue were identified by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. Two non-ionic detergents exhibiting little u.v. absorption were more efficient than u.v.-absorbing Triton X-100. Evidence is presented of an interchange between protei...

Acid dissociation equilibrium constants and rate constants for disulfide interchange reactions have been measured in 50% methanol at low ionic strength for peptides containing cysteines with local ionic neighboring groups. These physical constants may be correlated by separation of free energy contributions into solvent-independent and solvent-dependent factors. The former represent inductive e...

A direct current bridge with vacuum tube detector is described for measuring the resistance of living cells. Methods for evaluating the surface of contact with the protoplasm, and the leakage around the cell wall, allow us to calculate the effective resistance of the protoplasm. In Valonia ventricosa this is usually at least 10,000 ohms per square centimeter and is often much higher. This is in...

Key factors that mediate vascular smooth muscle cell proliferation and migration are platelet-derived growth factor (PDGF) and thrombospondin 1 (TSP1). We now report that PDGFBB bound tightly and specifically to TSP1, that this interaction was markedly dependent on the disulphide bond arrangement in TSP1, and that binding of PDGFBB to TSP1 did not preclude PDGFBB from binding to its receptor on...

The Poisson-Nernst-Planck system of equations used to model ionic transport is interpreted as a gradient flow for the Wasserstein distance and a free energy in the space of probability measures with finite second moment. A variational scheme is then set up and is the starting point of the construction of global weak solutions in a unified framework for the cases of both linear and nonlinear dif...