Leptospirosis caused by pathogenic species of the genus Leptospira is a re-emerging zoonotic disease, which affects a wide variety of host species and is transmitted by contaminated water. The genomes of several pathogenic Leptospira species contain a gene named invA, which contains a Nudix domain. However, the function of this gene has never been characterized. Here, we demonstrated that the i...

AIM The aim was to characterize Salmonella enterica serovar Gallinarum isolated from backyard poultry by polymerase chain reaction (PCR) detection of virulence genes invasion (invA) and Salmonella plasmid virulence C (spvC). MATERIALS AND METHODS Two strains of Salmonella serovar Gallinarum isolates used in this study were obtained from an outbreak of fowl typhoid in backyard Vanaraja fowl. P...

An invasion gene homolog, invA, of Rickettsia prowazekii has recently been identified to encode a member of the Nudix hydrolase subfamily which acts specifically on dinucleoside oligophosphates (Np(n)N; n >/= 5), a group of cellular signaling molecules known as alarmones. InvA is thought to enhance intracellular survival by regulating stress-induced toxic nucleotide levels during rickettsial in...

To understand further the molecular basis of rickettsial host cell invasion, Rickettsia prowazekii invasion gene homolog (invA) has been characterized. Our previous experiments have shown that InvA is an Ap5A pyrophosphatase, a member of the Nudix hydrolase family, which is up-regulated during the internalization, early growth phase, and exit steps during rickettsial mammalian cell infection. I...

A novel, rapid and simple fluorescence resonance energy transfer (FRET) based Salmonella specific gene, invA, detection system was developed, in which quantum dots (QDs) and graphene oxide (GO) worked as fluorescent donor and quencher, respectively. By measuring the fluorescence intensity signal, the Salmonella specific invA gene could be sensitively and specifically detected with a limit of de...

Detection of pathogenic Salmonella isolated from meat and poultry products by detecting virulence invA gene using PCR technique.A total of100 meat and poultry products samples were collected from shops and supermarkets; including 50 meat products samples and 50 poultry products. Salmonella was detected by conventional isolation methods and then PCR was carried out to detectinvA gene in isolated...

Many epidemiological studies of Salmonella rely on conventional bacteriological culture methods to detect Salmonella in fecal samples. These culture-based methods are inefficient for epidemiological studies in populations with a low prevalence of Salmonella. The objective of this study was to optimize a protocol that uses pooled Salmonella enrichment broth cultures of bovine feces and polymeras...

salmonella spp. are zoonotic enteric bacteria able to infect humans, livestock and wildlife. the aim of this study was to investigate the prevalence of salmonella (spp.) and to determine antibiotic susceptibility, serogrouping and presence of inva gene (salmonella invasion gene a) in the detected strains in wild captive herbivores in iran. the fecal samples of 103 animals from 8 different sp...

a total of 60 neck skin swab samples were taken from 12 different broiler flocks after the chilling stage of processing at a commercial broiler slughtering facility in mashhad. the presence of salmonella was assessed by conventional culture method and confirmed by using poly o and poly h antiserum in serotyping. pcr amplification of inva gene as a specific method for detection of salmonella was...

Accurate enumeration of Salmonella spp. is important for assessing whether this pathogen has survived composting. Recent literature has reported that enumeration of Salmonella spp. using standard microbiological methods has a numbers of disadvantages, particularly the time taken to obtain a result. This research is an attempt to develop a rapid, low-cost detection method that is quantitative, h...