The delivery of Public Services to citizens is becoming a challenging task, as urban populations increase and the related networks are becoming more complex and entangled. In this article, we present a platform designed for the optimal coordination of a city's public agencies and emergency response teams. Such coordination is particularly relevant in emergency situations when the infrastructure...

INTRODUCTIONThe most common way for a cell to respond to internal and external signals is to change its gene expression pattern. This requires the synchronization of regulatory steps along the expression pathway. Biological imaging techniques can be used to visualize and measure such processes in individual live cells in real time. This article discusses the use of a fluorescent RNA-binding pro...

INTRODUCTIONThe Antirrhinum species group comprises approximately 20 morphologically diverse members that are able to form fertile hybrids. It includes the cultivated snapdragon Antirrhinum majus, which has been used as a model for biochemical and developmental genetics for more than 75 yr. The research infrastructure for A. majus, together with the interfertility of the species group, allows A...

INTRODUCTIONThe tips of Drosophila testes harbor the germ line stem cells, their amplifying daughters, and the cells of the stem cell lineage environments. These cells, located within the apical 100 μm, are specifically hard to label, as the testes appear to be less penetrable at the tips. Here we report an in situ hybridization protocol that works reliably to detect RNA in the cells at the api...

University campuses must take a leadership position in helping cities and nations address the challenges and opportunities of climate change, urban population growth in developing countries, as well as aging populations in developed countries. To turn these challenges into opportunities require systems integration approaches to providing regional, urban and local energy, connectivity, water, mo...

The city of San Francisco in California has 826,000 residents and is growing slowly compared to other large cities in the western United States, facing concerns such as an aging population and flight of families to nearby suburbs. This case study investigates the social and demographic factors that are causing this phenomenon based on data that were collected by San Francisco’s city controller’...