mice are ideal models for icsi experiments because of the ease of culturing eggs/embryos in vitro and the availability of ample genetic information. unfortunately, it has been extremely difficult. in this study we improved the mouse icsi method by using a new holding pipette that was made of two pipettes such that one pipette was pulled and heat merged into the other one. the outer pipette had ...

the objective of this study was to compare the effectiveness of various combination activators of bovine oocyte following intracytoplasmic sperm injection (icsi) in terms of cleavage and embryo development. bovine icsi oocytes were treated with a variation of treatments of combined or single activators as follows; 30 v for 15 x 2 μsec of electrical pulse +10 μg/ml cycloheximide (chx),7% ethanol...

background: the etiologic cause in near one third of male factor infertility is unknown. the percentage of men with idiopathic infertility who have been successfully treated by the empirical therapeutic modalities is not high. objective: the aim of this study was to assay the effect of l-carnitine on sperm parameters in patients who needs intracytoplasmic sperm injection (icsi) as a method for ...

background: testis tissue xenografting and the resultant sperm in a xenograft may provide a unique approach to rescue the genetic material of males that die prematurely and is a model for the study of human spermatogenesis and can represent an alternative approach for fertility preservation in cancer patients. this study was aimed to evaluate the xenogenic dog sperm in formation of male pronucl...

background: pregnancy is a successful transplantation. the factors evading rejection of the fetus are poorly understood. recently an interest has grown, in hla-g molecules as one of these factors. since these antigens are mainly expressed on the surface of cytotro-phoblasts that are in direct contact with maternal tissues, it has been suggested that these molecules may have a role in induction ...

background: intracytoplasmic sperm injection (icsi) is an advanced technique employed in assisted reproductive clinics for treatment of infertile couples. the reproductive endocrinologists try their level best to identify factors that enhance success rate after icsi. objective: to compare estradiol progesterone ratio on ovulation induction day amongst pregnancy outcome groups following icsi. ma...

the purpose of this study was to evaluate the efficiency of intracytoplasmic sperm injection (icsi) and piezo-assisted sperm injection after pretreatment with calcium ionophore (cai) on the mouse embryo development. methods: in this study, the conventional icsi and piezo-icsi procedures were used. the efficacy of the methods was examined after mouse matured oocytes were fertilized with or witho...

background: improvement of assisted reproductive technique (art) results in higher pregnancy rates from positive beta hcg to take home baby statistics. despite developments in culture media allowing blastocyst stage transfer, some centers apply second, third and sometimes fourth day post injection for embryo transfer. this study aimed to compare their reproductive outcomes.   methods : this pro...