bioavailability of three rumen protected methionine (met) sources with different protection methods (mepron® m85, evonik industries, germany; methioplus®, soda nutrition, italy and methilock®, tehrandaneh co. iran) were evaluated in 2 experiments with 6 canulated non-lactating holstein cows. in experiment 1, the ruminal in situ and mobile bag techniques were used for assessing ruminal degradabi...

The disappearance of dry matter (DM), crude protein (CP), and amino acids (AA) in steers after rumen incubation and intestinal passage of alfalfa hay, barley hay, corn silage, barley grain, corn grain, wheat bran, meat meal, fish meal, cottonseed meal, and soybean meal were measured in 3 steers using a mobile nylon bag technique. Ruminal degradation of individual AA differed between feedstuffs....

The objective was to assess the ability of the in situ mobile nylon bag method for predicting small intestinal and total tract starch digestibility. Starch disappearance was measured for 18 samples of different cereals and legumes subjected to different physical and chemical processing methods and compared with coherent in vivo digestibility. Starch disappearance was measured both with and with...

Steers (379 +/- 10 kg) with ruminal, duodenal, and ileal cannulas were used in a 5 x 5 Latin square digestion trial to quantify and evaluate the relationship between intestinal protein supply and intestinal starch disappearance. Treatments were infusions of 0, 50, 100, 150, or 200 g/d of casein along with 1,042 g/d of raw cornstarch. Abomasal infusions were accomplished by passing tubing and a ...

Intestinal metabolism of dietary essential amino acids and its potential impact on their availability for whole-body growth has been the focus of many investigators' research over several decades. One of the major questions was concerned with the discrepancy between the disappearance of dietary essential amino acids from the small intestinal lumen and appearance in the portal circulation despit...

Eight Holstein steers (four at 300 kg, four at 406 kg) fitted with an elevated carotid artery, hepatic portal and mesenteric venous catheters, and abomasal and ileal cannulas were used in several 4 x 4 Latin square experiments to evaluate small intestinal starch digestion. They were fed alfalfa hay at 1.5% of BW and abomasally infused with water or glucose, corn starch or corn dextrin (one carb...

The disappearance of total N, non-protein-N and amino acid-N after washing, rumen incubation and intestinal passage of sugarbeet pulp, maize-gluten feed, maize feed meal, palm kernel meal, soyabean hulls, soyabean meal, grass silage, maize silage and concentrate was measured in four dairy cows using nylon-bag techniques. Disappearance of amino acid-N after washing varied between feedstuffs from...

[14C]choleragen was used to study the rate of disappearance of choleragen enterotoxin from the jejunum of rats. [14C]bovine serum albumin (BSA) was studied in a similar manner. Almost one-third of the labeled toxin had disappeared from the intestine after 6 h. Its rate of disappearance was the same in germfree rats as in conventional rats. The rate of proteolysis of [14C]choleragen and [14C]BSA...

this study was carried out to determine the effects of gamma irradiation on dry matter (dm) and on crude protein (cp) degradation of rapeseed meal, using nylon bags and sds-page techniques. ruminal nylon bags of either untreated or irradiated rapeseed meal at doses of 25, 50 and 75 kgy were suspended in the rumen of four non-lactating holstein cows for up to 48 h. intestinal crude protein diges...