In order to investigate the influence of aflatoxin B1 (AFB1) on intestinal bacterial flora, 24 Kunming mice (KM mice) were randomly placed into four groups, which were labeled as control, low-dose, medium-dose, and high-dose groups. They were fed intragastrically with 0.4 mL of 0 mg/L, 2.5 mg/L, 4 mg/L, or 10 mg/L of AFB1 solutions, twice a day for 2 months. The hypervariable region V3 + V4 on ...

AIM To study the role of intestinal flora in inflammatory bowel disease (IBD). METHODS The spatial organization of intestinal flora was investigated in normal mice and in two models of murine colitis using fluorescence in situ hybridization. RESULTS The murine small intestine was nearly bacteria-free. The normal colonic flora was organized in three distinct compartments (crypt, interlaced, ...

Entamoeba histolytica was first cultured in vitro in 1925 (Boeck and Drbohlav, 1925) but has not yet been grown in the absence of living bacteria. Experimentally the dependence of E. histolytica on living bacteria in the intestine is well illustrated by the inability of E. histolytica to survive and invade the bowel wall of germ-free guinea-pigs (Phillips, Wolfe, Rees, Gordon, Wright, and Reyni...

The present results of investigations performed on fresh fecal samples from a healthy adult and subjects with acute leukemia cultured bacteriologically using both aerobic and anaerobic techniques have led to the following conclusions. 1) In studies on the intestinal bacterial flora of the subjects examined, obligate anaerobic bacteria rather than facultative anaerobes accounted for more than 99...

The gastrointestinal tract has various functions including digestion, the production of hormones with local and systemic effects, a major role in immunological function, and acting as a barrier against antigens within its lumen. The intestinal microflora is an ecosystem which harbours over 400 bacterial species, predominantly anaerobes which outnumber facultative anaerobes. Most flora is presen...

The composition of the human intestinal flora is important for the health status of the host. The global composition and the presence of specific pathogens are relevant to the effects of the flora. Therefore, accurate quantification of all major bacterial populations of the enteric flora is needed. A TaqMan real-time PCR-based method for the quantification of 20 dominant bacterial species and g...

Bacterial infection, especially with intestinal-type bacterial flora, is a common complication in patients with cirrhosis [1–4]. Spontaneous bacterial peritonitis (SBP), urinary tract infection, pneumonia, spontaneous bacterial empyema and bacteraemia are the most frequent infective complications in this group. The incidence of infection with Gram-positive cocci, in particular, has increased in...

The association between worm infections and bacterial diseases has only recently been emphasized. This study examined the effect of experimental Angiostrongylus costaricensis infection on endogenous intestinal flora of Swiss Webster mice. Eight mice aging six weeks were selected for this experiment. Four were infected with A. costaricensis and the other four were used as controls. Twenty eight ...