conclusions real-time pcr and elisa showed high levels of production of 1a/jfh1 chimeric hcv in the huh7.5 cell culture. the constructed virus can be used for future studies, including the development of new hcv drugs and vaccines. background the e2 glycoprotein is an important encoded hepatitis c virus (hcv) protein that contains three different variable regions. objectives the aim of the pres...

The hepatitis C virus glycoprotein E2 receptor-binding domain is encompassed by amino acids 384 to 661 (E2(661)) and contains two hypervariable sequences, HVR1 and HVR2. E2 sequence comparisons revealed a third variable region, located between residues 570 and 580, that varies widely between genotypes, designated here as igVR, the intergenotypic variable region. A secreted E2(661) glycoprotein ...

Little is known about the structure of the envelope glycoproteins of hepatitis C virus (HCV). To identify new regions essential for the function of these glycoproteins, we generated HCV pseudoparticles (HCVpp) containing HCV envelope glycoproteins, E1 and E2, from different genotypes in order to detect intergenotypic incompatibilities between these two proteins. Several genotype combinations we...

BACKGROUND The E2 glycoprotein is an important encoded hepatitis C virus (HCV) protein that contains three different variable regions. OBJECTIVES The aim of the present study was to construct an HCV 1a/JFH1 chimeric virus by replacing the intergenotypic variable region (igVR) fragment of the highly variable region of the E2 gene of the Japanese Fulminant hepatitis genotype 2a JFH1 virus with ...

OBJECTIVES DNA sequencing is the gold standard for hepatitis B virus (HBV) genotyping. We investigated the intergenotypic discriminatory capabilities of various target gene regions over the entire HBV genome, introducing a novel data evaluation approach generally applicable in viral genotyping. METHODS Complete genome sequences of seventy HBV variants obtained from the sera of 50 Syrian patie...

The three variable regions of hepatitis C virus (HCV) glycoprotein E2 can be removed simultaneously from the E2 ectodomain (residues 384-661) without affecting folding or CD81 binding. In this study, we show that deletion of hypervariable region (HVR) 2 or the intergenotypic variable region (igVR) in the context of the E1E2 polyprotein eliminates formation of heterodimers, reduces CD81 binding ...

To address the need for broad-spectrum antiviral activity characterization of hepatitis C virus (HCV) polymerase inhibitors, we created a panel of intergenotypic chimeric replicons containing nonstructural (NS) protein NS5B sequences from genotype 2b (GT2b), GT3a, GT4a, GT5a, and GT6a HCV isolates. Viral RNA extracted from non-GT1 HCV patient plasma was subjected to reverse transcription. The N...

Background. Chronic hepatitis B (CHB) remains a severe global public health concern. Hepatitis B virus (HBV) can be divided into 8 genotypes with different geographical distribution and virological features. We demonstrated genotypes B and C prevalent in southern and northern China, respectively, had divergent genotype-dependent amino acid polymorphisms and variations in reverse transcriptase (...