An efficient Agrobacterium-mediate transformation method was developed by employing different duration of sonication, Agrobacterium strains, type of inoculation medium and concentrations of acetosyringone in Arya cultivar of wheat. Immature embryos were used as an explant for inoculation with Agrobacterium tumefaciens harboring the recombinant pBI121 plasmid. Among the durations of sonication t...

To identify the most rapid and reliable technique for recovery and identification of Staphylococcus aureus small-colony variants (SCVs), the colonial appearance of 106 isolates representing SCVs and the normal phenotype were evaluated on two newly described chromogenic agar media. Although almost all of the SCVs grew on the chromogenic agar media, they did not exhibit a change of color. In comp...

OBJECTIVE This study aimed to evaluate the normal flora of conjunctiva and lid margin, as well as its antibiotic sensitivity. DESIGN This was a prospective cross-sectional study. PATIENTS AND METHODS A prospective study was conducted on 120 patients who underwent cataract surgery at the Phramongkutklao Hospital from September 2014 to October 2014. Conjunctival and lid margin swabs were obta...

Rectal/vaginal specimens (n = 97) were collected in parallel using ESwab and Liquid Stuart (LS) rayon fiber wrapped swab collection devices. Each collection device was used to directly inoculate culture medium and LIM broth. Medium inoculation by ESwab was conducted using the Walk-Away specimen processor (WASP). Medium inoculation by the LS device was conducted manually. The sensitivities of ES...

We compared the use of universal transport medium and eSwab transport medium held at room temperature or 37 degrees C to bedside inoculation and immediate incubation of culture media for the detection of Trichomonas vaginalis. There were no significant culturable differences in the sensitivity of either of the transport media to that of bedside inoculation.

During the 58 years since Muhlens and Hartmann (1906) first reported the cultivation of anaerobic Treponemataceae in vitro, little has been accomplished in classifying these organisms other than by morphological description. A major obstacle to systematic classification has been the difficulty encountered in cultivating these organisms in general and as surface colonies in particular. Breed, Mu...

seedling characteristics of different sunflower (helianthus annus l.) cultivars under drought stress and inoculation with the azospirillum lipoferum in a spilt-factorial layout based on randomized complete block design with three replications were evaluated. treatments included dehydration stress (seed produced on maternal plants which irrigated after 60 (desirable irrigation), 120 (medium stre...

This research has been done to examine the effect of bio-priming on germination parameters related to seeds of canola cultivar (Hayola 401) under high salinity as factorial in seed laboratory of Shahed University based on a Completely Randomized Design (CRD) in three replications. Experimental factors included bio-primin...

BACKGROUND In general practice, infectious conjunctivitis is a common and mostly (64%) self-limiting disorder. In case of an aberrant course or severe symptoms, a general practitioner may take a culture. Direct inoculation is considered the reference standard, but usually a swab is sent to a laboratory. OBJECTIVES To compare the diagnostic performance of the swab, transported by surface mail ...

Two ciliate protozoans, Stylonychia mytilus and Paramecium caudatum isolated from tannery effluent and cultured in a medium containing inorganic salts showed tolerance against endosulfan (1.8 μg/ml). The protozoans uptake the insecticide from the medium for utilization. Stylonychia mytilus reduced 21% endosulfan from the medium within 48 hours of inoculation of insecticide containing medium wit...