insect immunity comprises behavioral, physical and innate immunity. in this study, innate immune response of the greater wax moth larvae, galleria mellonella against two species of entomopathogenic nematodes, heterorhabditis bacteriophora and steinernema feltiae,   as important biocontrol agents were studied. total haemocyte count in larvae injected with h. bacteriophora increased immediately. ...

introduction: stored product pests are a major problem in the storage of agricultural products that cause damage from harvest until consumption. greater wax moth (galleria mellonella l.) is one of the most important pests of stored products and beehives. the most common method to control this pest in many countries is use of chemical compounds. however, these compounds have disadvantages such a...

Galleria mellonella (greater wax moth or honeycomb moth) has been introduced as an alternative model to study microbial infections. G. mellonella larvae can be easily and inexpensively obtained in large numbers and are simple to use as they don't require special lab equipment. There are no ethical constraints and their short life cycle makes them ideal for large-scale studies. Although insects ...

Conventional assays for quantifying the virulence of microbial pathogens and mutants have traditionally relied upon the use of a range of mammalian species. A number of workers have demonstrated that insects can be used for evaluating microbial pathogenicity and provide results comparable to those that can be obtained with mammals since one component of the vertebrate immune system, the innate ...

The cellular and humoral immune reactions in haemolymph of the wax moth Galleria mellonella larvae naturally injected by venom of ectoparasitic wasp Habrobracon hebetor were analyzed. A strong decline of phenoloxidase (PO) activity in the haemolymph and the number of haemocytes with PO activity of envenomated wax moth was observed. In addition, it has been shown that the rate of capsule melaniz...

rearing methods for bracon hebetor (say) (hym., braconidae) were investigated in the series of laboratory experiments designed to enhance the yield of the mass rearing of this parasitoid for biological control of lepidopteran field and stored product pests. in these experiments, the effects of parasitoid and host densities on fertility and sex ratio of b. hebetor were assessed. in parasitoid de...

microRNAs (miRNAs) coordinate several physiological and pathological processes by regulating the fate of mRNAs. Studies conducted in vitro indicate a role of microRNAs in the control of host-microbe interactions. However, there is limited understanding of miRNA functions in in vivo models of bacterial infections. In this study, we systematically explored changes in miRNA expression levels of Ga...

The role of protein kinase A (PKA) in the humoral immune response of the greater wax moth Galleria mellonella larvae to live gram-positive bacteria Micrococcus lysodeikticus and gram-negative bacteria Escherichia coli was investigated. The immune challenge of larvae with both kinds of bacteria caused an increase in fat body PKA activity depending on the injected bacteria. Gram-positive M. lysod...

Abstract Staphylococcus haemolyticus is a normal inhabitant of healthy skin and also an emerging cause nosocomial infections in the immunocompromised. S. often lead to bacteremia, septicemia, peritonitis, endocarditis various other outcomes. The goal this project was establish invertebrate infection model for studying innate immune response using wax moth larva (Galleria mellonella). We have fo...