capillary electrophoresis (ce) with indirect uv detection is an interesting analytical method for the analysis of drugs and pharmaceuticals. good and reproducible capillary quality is needed to develop robust methods and to facilitate method transfer in ce. it is widely accepted that preconditioning procedures are indispensable in capillary electrophoresis in order to achieve reproducibility of...

The branched-chain amino acids (BCAAs) including leucine (Leu), isoleucine (Ile) and valine (Val) play a pivotal role in the human body. Herein, we developed capillary electrophoresis (CE) coupled with conventional UV detector to quantify underivatized BCAAs in two kinds of sport nutritional supplements. For direct UV detection at 195 nm, the BCAAs (Leu, two enantiomers of Ile and Val) were sep...

Aminoglycoside antibiotics can be determined by capillary zone electrophoresis (CZE) with indirect UV detection in the anionic mode with a reversed electroosmotic flow (EOF) by addition of FC 135 to the background electrolyte. The effective mobilities of thirteen aminoglycoside antibiotics were determined as a function of pH. Applying CZE with indirect UV detection in the anionic mode and rever...

easily degradating and various isomeric forms of rapamycin (sirolimus) face the determination of this compound to many challenges. in this study, we developed and validated the isocratic reversed phase high performance liquid chromatographic (rp-hplc) method for rapamycin. separation was performed on a c8 column (mz, 15 × 4.6 mm, 5 µm particle size) using methanol:water (80:20 v/v) as the mobil...

a gradient reversed-phase high performance liquid chromatography (hplc) method was developed for the assay of cetrorelix acetate, a synthetic decapeptide with gonadotropin-releasing hormone (gnrh) antagonistic activity used in infertility treatment. the hplc method, which is used to determine cetrorelix in bulk and pharmaceutical dosage forms, was validated per ich guidelines. the chromatograph...

the present study was designed to develop a simple, validated liquid chromatographic method for the analysis of azithromycin in bulk and pharmaceutical dosage forms using ultraviolet detector. the best stationary phase was determined as c18 column, 5 µm, 250 mm × 4.6 mm. mobile phase was optimized to obtain a fast and selective separation of the drug. flow rate was 1.5 ml/min, wavelength was se...