potato virus y (pvy) and potato leafroll virus (plrv) are two major viruses which reduce 10-90 percent of yield and quality of potato tubers. in the present study, the efficiency of different methods, including meristem culture, thermotherapy followed by meristem culture and electerotherapy followed by meristem culture in two cultivars (agria and marfona) infected by plrv or pvy were evaluated....

elimination of potyviruses from two commercial iranian garlic populations, hamedan and azar-shahr, through thermotherapy and by meristem tip culture were investigated. the infected bulbs of either one of the populations were initially treated at 30o c for one week and then at 36oc for 5 weeks. the meristems of cloves at two sizes of 0.4-0.6 mm and 0.6-0.8 mm were then cultured on ms medium supp...

There is great interest in the phenotypic, genetic and epigenetic changes associated with plant in vitro culture known as somaclonal variation. In vitro propagation systems that are based on the use of microcuttings or meristem cultures are considered analogous to clonal cuttings and so widely viewed to be largely free from such somaclonal effects. In this study, we surveyed for epigenetic chan...

Production of genetically and phenotypically stable plantlets is the main purpose in commercial strawberry tissue culture. In this study, different tissues of Fragaria ananassa cv. Camarosa including stipule, apical meristem, leaf and petiole were cultured in Murashige and Skoog (MS) medium supplemented withdifferent concentrations of N6-benzyladenine (BA) (0.5, 1, ...

Bananas that provide a staple food to the millions of people are adversely affected by several viruses such as Banana bunchy Top Virus (BBTV), Banana Streak Virus (BSV), and Cucumber Mosaic Virus (CMV). These viruses are known to have a devastating effect on crop production and constraint to the international exchange and conservation of banana germplasm-a cornerstone for breeding new cultivars...

bean yellow mosaic virus (bymv, potyvirus, potyviridae) causes serious disease in gladiolus spp. in this work, the possibility of obtaining bymv free plant material from virus infected gladiolus corms was studied. thermotherapy, meristem-tip culture and combination of both techniques on infected corms/meristem-tip explants (0.5–1 mm in length) resulted in bymv elimination up to 15.38, 78.04 and...

Chrysanthemum stunt viroid (CSVd) can infect Argyranthemum and cause serious economic loss. Low temperature treatment combined with meristem culture has been applied to eradicate viroids from their hosts, but without success in eliminating CSVd from diseased Argyranthemum. The objectives of this work were to investigate (1) the effect of low temperature treatment combined with meristem culture ...

Pure cultures of a gram-negative, rod-shaped bacterium, which produced endospores after 3 to 5 days on solid medium, were isolated exclusively from tissue cultures of the date palm Phoenix dactylifera L. Electron microscopic examination of thin sections of the bacteria revealed the bilayer membrane typical of gramnegative bacteria and confirmed the nature of the spores as true endospores. Bioch...