rate of in vitro maturation of oocytes is one of the challenges of assisted reproductive techniques. in this study we investigated the effects of supplementation of cysteamine on the rate of in vitro maturation of oocytes in two different media. germinal vesicle oocytes were collected from mouse ovary and cultured in two media (tcm199 and meme) with 0, 50, 100, 200, 500 µm/ml cysteamine. number...

in vitro maturation (ivm) is a method that immature oocytes in antral follicles are extracted and matured in laboratry conditions. this review has attempted to provide the current knowledge and recent findings in in vitro maturation of oocytes and highlights the most important factors involved in this process. the review is based on literature reports and the author’s experience. in ivm cycles,...

background: it is important to protect oocytes and embryos from oxidative stress in the culture medium. melatonin has been shown to be a direct free radical scavenger. objective: effect of melatonin during in vitro oocyte maturation, fertilization and embryo development of mouse oocytes was evaluated. materials and methods: oocytes from supper-ovulated mouse were divided to two groups: cumulus ...

the use of ovarian stimulation for infertility treatment is associated with side effects of ovarian hyperstimulation syndrome (ohss) and potential cancer risk. this is also true in high risk women such as those polycystic with ovary (pco) and polycystic ovarian syndrome (pcos). in vitro maturation (ivm) of oocytes was primarily developed to make ivf safe for women with pco and at high risk of o...

background: addition of amino acids to the culture medium is beneficial for embryonic development in many species. objective: the objective of this study was to investigate the effects of amino acids on the in vitro maturation and embryonic development of the bovine oocyte. materials and methods: bovine ovaries were collected from a local abattoir and brought into laboratory. cumulus-oocyte com...

background: in vitro maturation (ivm) of oocytes is a promising technique to reduce the costs and avert the side-effects of gonadotropin stimulation for in vitro fertilization (ivf). the pregnancy rates from oocytes matured in vitro are much lower than those of in vivo stimulation cycles, indicating that optimization of ivm remains a challenge. objective: in this study, we investigated the effe...

background   objective:   materials and methods   results   conclusion   key words:   . vitrification, viability, in vitro maturation, gvbd, oocyte, mouse : these results suggest that step-wise vitrification of gvbd oocytes as compared to single step vitrification was better in the rate of survival and in vitro maturation of oocytes. : all non-vitrified oocytes were viable after 24 hr; however,...

background to current knowledge, different oocyte's recovery method and various seasons have profound impact on in vitro embryo production (ivep). objectives the aim of this study was to define an efficient recovery method for oocytes harvesting from slaughterhouse material in different seasons, and their effects on ivep yield. materials and methods ovaries from slaughtered ewes in breeding sea...

Background: Oocyte maturation is usually defined as the period of progression from the first to the second meiotic arrest and involves coordinated nuclear and cytoplasmic modification. Ca2+ signals are known as important regulators of oocyte maturation. Extracellular Ca2+ is important for first polar body formation, normal meiosis kinetics, and for preserving the chromosome and spindle configur...