materials and methods in this descriptive study, 2988 stool samples of patients with acute gastroenteritis were collected from children’s hospitals of 5 main cities of iran. the samples were sent in frozen condition to pediatric infection research center in tehran and stored at -70°c. elisa test was performed for detection of rotavirus antigens. the mean age of study population was 1 to 5 years...

conclusions: it was concluded that the elisa is a suitable method for large scale screening of antibodies against aspergilloma. the easy techniques have shortened the required time of tests (approximately 1.30 hours for our elisa, as opposed to 2.30 hours with commercial elisa kit). this may be a substantial benefit for large numbers of samples that should be tested. materials and methods: an i...

background: the ability to sensitively detect vibrio cholera with pcr-elisa method represents a considerable advancement over alternative more time-consuming methods for detection of this pathogen. the aim of this research is to evaluate the suitability of a pcr-enzyme-linked immunosorbent assay for sensitive and rapid detection of v. cholera o1. methods: the 398-bp sequence of a gene that code...

background: nuts are one of the main consumed snacks worldwide and also have an important role among iranian's food habits. natural contamination of nuts with aflatoxin is unavoidable and causes a special challenge for nuts safety and quality. objectives: the purpose of this research was to study the aflatoxin contamination in commercially-available nuts (pistachio, walnut and peanut) in the ma...

b ackground : tuberculosis is a crucial health problem. establishing a rapid, reliable and still inexpensive diagnostic method for tuberculosis seems to be substantial in developing countries where tb has very high incidence rate . methods:   an indirect  enzyme-linked  immunosorbent  assay (elisa )   was established to detect serum antibodies against mycobacterium  tuberculosis. three kinds of...

conclusions afm1 contamination of milk is not a concern in this city. however, dairy cattle feed samples of various livestocks must be regularly checked for aflatoxin and the storage conditions of feeds should be strictly controlled. results afm1 was detected in all milk samples. the mean concentration of afm1 was 11.61 ± 0.72 ng/l with a range of 6.3-23.3 ng/l. none of the samples had levels e...

background: timely diagnosis of leptospirosis is essential for early and effective treatment, for there are many differential diagnoses for it.. leptospiral researchers have an increasing interest in developing new serological methods with recombinant antigens to improve the leptospirosis diagnosis. several serological tests have been developed for the proper diagnosis of leptospirosis. objecti...

objectives: leptin is a hormone-like protein produced by the adipose tissue. it plays an important role in protection of host against inflammation and infection. some studies have reported changes in leptin levels in the gingival crevicular fluid (gcf), saliva and blood serum of patients with periodontal disease compared to healthy individuals. the aim of the present study was to compare the sa...

background: aflatoxin m1 (afm1) as a carcinogenic and toxic compound has considerable significance from public health viewpoint. the aim of this study was to determine the level of afm1 in pasteurized and uht milk in west-azerbaijan province of iran. methods: a total of 360 commercial milk samples including 220 pasteurized milk and 140 ultra-high-temperature (uht) milk were purchased randomly f...

cholera toxin b subunit (ctb) has been extensively studied as an immunogen, adjuvant, and inducer of oral tolerance in many investigations. production of ctb has been carried out in the bacterial, plant, insect and yeast expression systems. in this study the expression of the ctb containing a 6xhis-tagged was performed by escherichia coli (e.coli) m15. the yield of purified pentameric recombina...