tuberculosis is a chronic mycobacterial infection. the main effector cells against mycobacterium tuberculosis are cd4+ t lymphocytes. our objective in this research was to evaluate the quantity of t lymphocytes and their subpopulations before and after treatments with combination of 4 drugs (rifampcin, isoniaside, pyrasinamide, ethambutal) for 2 months directly in sputum-positive tuberculosis p...

The evolution of cellular and molecular immunology has made available high-throughput discovery tools which can provide an entirely new, comprehensive and multidimensional picture of the immune system. A combination of different approaches, such as deep phenotyping by mass and flow cytometry, multiplex gene expression and functional assays, have been applied to identify immunological and epigen...

Key points Traditionally the female sex, compared with male has been perceived as having greater variability in many physiological traits, including within immune system. We investigated effects of biological sex and reproductive cycle on numbers circulating leukocytes C57BL/6J mice. show that but not cyclicity, a significant effect peripheral blood cell prevalence variability, differences were...

background: dysregulation of wnt signaling has been reported in many malignancies. objective: this study was conducted to investigate the expression pattern of 14 members of the wnt gene family in different immunophenotypic subtypes of all. methods: semi-quantitative rt-pcr was performed on samples from 71 all patients and 36 age-matched healthy individuals. the all patients were categorized in...

The RAM immunophenotype has been recently described as a subtype of acute myelogenous leukemia (AML) that is characterized clinically by extremely poor prognosis. We present a case of AML with RAM immunophenotype in a 5-year-old patient that resulted in poor outcome despite early hematopoietic cell transplant. We describe the unusual morphologic features that, along with the distinct immunophen...

Adult T-cell leukemia/lymphoma (ATL), a human T-lymphotropic virus type 1 (HTLV-1)-associated disease, has a highly variable clinical course and four subtypes with therapeutic and prognostic implications. However, there are overlapping features between ATL subtypes and between ATL and nonmalignant (non-ATL) HTLV-1 infection complicating diagnosis and prognostication. To further refine the diagn...

Background and Objectives. Detection of minimal residual disease (MRD) by multiparameter flow cytometry is an emerging prognostic factor in patients with acute myeloid leukemia (AML). The present analysis aimed at improving the applicability of this approach to more patients with AML. Design and Methods. Bone marrow samples from unselected patients with AML at diagnosis and from healthy volunte...

Burkitt lymphoma (BL) exhibits a characteristic immunophenotype that is positive for pan-B-cell antigens and CD10 and expresses clonal surface immunoglobulins (SIgs). We evaluated 35 BLs and identified atypical immunophenotypes in 4 including SIg light chain negativity in 4, negativity for B-cell antigens in 2, lack of CD10 in 1, and CD4 expression in 1. All 4 cases showed morphologic features ...