Ephexin-1 functions as guanine nucleotide exchange factors for the Rho-type GTPases which have important roles in neuronal development including axon guidance, migration, morphogenesis, and plasticity of neurons. As little is known about ephexin-1 in the cerebellum, we investigated the immunolocalization of ephexin-1 in the developing canine cerebellum. While the cellular maturation was followe...

A simple method is described for high-resolution light and electron microscopic immunolocalization of proteins in cells and tissues by immunofluorescence and subsequent photooxidation of diaminobenzidine tetrahydrochloride into an insoluble osmiophilic polymer. By using eosin as the fluorescent marker, a substantial improvement in sensitivity is achieved in the photooxidation process over other...

1. A liver canalicular plasma-membrane fraction enriched 115-155-fold in five marker enzymes relative to the tissue homogenate was obtained by sonication of liver plasma membranes followed by fractionation in iso-osmotic Nycodenz gradients. 2. Two lateral-plasma membrane fractions were also collected by this procedure; the lighter-density fraction was still associated with canalicular membranes...

in order to determine the effects of fish size and weight on its salinity tolerance, the chloride cells (ccs) immunolocalization changes were examined in caspian salmon (salmo trutta caspius) parrs, with the same age (about 2 years old) but different types (type a: 4.88 g, 8.36 cm; type b: 14 g, 11.84 cm; type c: 24.05 g, 14.08 cm). fish survival rate, blood osmolality, gills ccs histological a...

the ultrastructure of the cells, na+, k+-atpase activity and immunolocalization were examined in the barnchial chamber of libellula lydia (drury, 1773) larvae. na+,k+-atpase activity and localization were performed through biochemical techniques and immunofluorescence light microscopy using a mouse monoclonal antibody iggα5, respectively. the branchial chamber possesses six pair gills lamellae ...

This report describes the molecular characterization of the Tc8.2 gene of Trypanosoma cruzi. Both the Tc8.2 gene and its encoded protein were analyzed by bioinformatics, while Northern blot and RT-PCR were used for the transcripts. Besides, immunolocalization of recombinant protein was done by immunofluorescence and electron microscopy. Analysis indicated the presence of a single copy of Tc8.2 ...

Redling, Stephanie, Imke L. Pfaff, Michael Leitges, and Volker Vallon. Immunolocalization of protein kinase C isoenzymes , I, II, , and ε in mouse kidney. Am J Physiol Renal Physiol 287: F289–F298, 2004. First published March 23, 2004; 10.1152/ajprenal. 00273.2003.—Localization of protein kinase C (PKC) isoenzymes , I, II, , and ε was studied employing Western blot analysis and immunohistochemi...

HENRIK VORUM, TAE-HWAN KWON, CHRISTIAAN FULTON, BRIAN SIMONSEN, INYEONG CHOI, WALTER BORON, ARVID B. MAUNSBACH, SØREN NIELSEN, AND CHRISTIAN AALKJÆR Department of Medical Biochemistry, Department of Cell Biology, Institute of Anatomy, and Department of Physiology, University of Aarhus, DK-8000 Aarhus C, Denmark; and Department of Cellular and Molecular Physiology, Yale University, New Haven, Co...